Kit for rapid identification of nocardia and using method of kit

A Nocardia and kit technology, applied in the field of differential diagnosis kit research and development, can solve the problems of false positive, high false negative rate, large human error, etc.

Inactive Publication Date: 2017-05-31
GUIZHOU MEDICAL UNIV
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Classical methods such as agarose gel electrophoresis are used to observe and analyze the test results, with large human errors, high false positive and false negative rates

Method used

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  • Kit for rapid identification of nocardia and using method of kit
  • Kit for rapid identification of nocardia and using method of kit
  • Kit for rapid identification of nocardia and using method of kit

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0019] 1. Materials and methods

[0020] 1.1 Experimental strains and reagents

[0021] One Nocardia strain CMCC(F)D4C, one Rhodococcus strain CMCC(F)D4B and one Gordonia strain IFM10866 were donated by the Department of Dermatology, Shanghai Changzheng Hospital and the Institute of Microbiology, Chinese Academy of Sciences.

[0022] 1.2 Instruments

[0023] ABI StepOnePlus real-time fluorescent quantitative PCR instrument is a product of Thermo Fisher Scientific (China) Co., Ltd.

[0024] 1.3 Design and synthesis of molecular beacon probes

[0025] According to the requirements of general hybridization probe design, according to the Nocardia strains published on GenBank secA1 For the gene sequence, molecular beacon probes were designed using Primer software, and the selected 19bp DNA fragment was homologously compared with the genomic DNA sequence of Nocardia strains, and it was confirmed that it was a specific DNA without homologous fragments. According to the requiremen...

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Abstract

The invention discloses a kit for rapid identification of nocardia. The kit is characterized in that a probe design sequence is shown as FAM-CCTAGA TGCGCTTCTTGATGTCGAC TCTAGG-DABCYL; and composition of the kit is shown as the Description. In accordance with the design, identification and diagnosis can be conducted on the nocardia without sequencing, so that the rapid identification of pathogenic actinomycetes is achieved, difficulties and bottlenecks in the identification and the diagnosis are solved, identification cost is reduced and operations are simplified; and in addition, influence to a PCR amplification reaction is avoided.

Description

technical field [0001] The invention relates to a kit for quickly identifying Nocardia and a method for using the kit, belonging to the field of research and development of differential diagnosis kits. Background technique [0002] Nocardia is an aerobic actinomycete, a saprophytic bacterium in soil, not a normal flora in humans and animals. Nocardia infection can not only occur after trauma in healthy people, but also cause fatal infection in immunocompromised patients. When inhaled or entered into the human body by trauma, it can cause infection of the lungs and subcutaneous tissue, and can also spread to the whole body, causing Infection of various internal organs. There are at least 25 species of pathogens in the genus Nocardia that treat human or animal diseases. Of the Nocardia isolated from the human body, Nocardia astratus accounts for 90%, and Nocardia brasiliensis accounts for 7%. Nocardiella guinea pigs accounted for 3%. It has been reported that Nocardia aster...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12R1/01
CPCC12Q1/6851C12Q1/689C12Q2531/113C12Q2563/107
Inventor 康颖倩王颜颜
Owner GUIZHOU MEDICAL UNIV
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