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Method for separating intestinal flora from human faeces

A technology of intestinal flora and feces, applied in the direction of bacteria, etc., can solve the problems of easy contamination and cross-contamination of open vessels, affecting the acceptance of medical workers to patients, and many impurities and pigments in intestinal flora liquid. Achieve the effect of reducing the chance of pollution and sample loss, good separation and extraction effect, and ensuring ecology and activity

Active Publication Date: 2017-06-09
CHONGQING BEIZHITANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The intestinal flora liquid prepared by these crude methods has many impurities and pigments, and has poor senses; the open containers used in the preparation process are prone to contamination and cross-contamination, and the preparation operation also brings sensory and psychological stress to the staff. Discomfort caused by a strong negative impact
Therefore, using crude methods to separate, extract and prepare intestinal flora liquid, whether it is the operation process or the quality of the product, has seriously affected the application of medical workers and the acceptance of the project by patients, hindering and Delayed clinical application of intestinal flora fluid

Method used

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  • Method for separating intestinal flora from human faeces
  • Method for separating intestinal flora from human faeces
  • Method for separating intestinal flora from human faeces

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A method for isolating intestinal flora from human feces, which specifically follows the steps below:

[0049] 1) Aseptic sampling

[0050] Sampling with sterilized double-layer sampling bags, such as figure 1 and 2Shown, this double-deck sampling bag 1 is inner and outer two layers 2,3, and bag body top is provided with a tear opening 4; The bottom of the double-layer sampling bag 1 is folded upwards, and the bottom end 9 is fixed on one side of the outer wall of the inner layer 2; a tear 4 is also provided on the bottom of the inner layer 2; the upper part of the double-layer sampling bag 1 is provided with a sealing strip 5 and Tear opening 6, and tear opening 6 is located at the top of sealing strip 5; the sampling operation is: tear the tear opening 6 of the sampling bag, seal it with sealing strip 5 after sampling, and send it to the experiment within 2 hours in a refrigerated environment room;

[0051] 2) Aseptic sample addition

[0052] Tear the tear 4 on t...

Embodiment 2

[0064] A method for isolating intestinal flora from human feces, which specifically follows the steps below:

[0065] 1) Aseptic sampling

[0066] Sampling with sterilized double-layer sampling bags, such as Figure 5 and 6 As shown, the double-layer sampling bag 1 is an inner and outer layer 2, 3, and a tear opening 4 is provided on the upper part of the bag body; The end 9 is fixed on the outer wall of one side of the inner layer 2; a tear opening 4 is also provided on the lower part of the inner layer 2; the sampling operation is: directly sample with the double-layer sampling bag 1;

[0067] 2) Aseptic sample addition

[0068] Tear the tear 4 on the upper part of the double-layer sampling bag, discard the outer layer 3, put the inner layer 2 into the sterile homogeneous filter bag 7, tear the tear 4 on the lower part of the inner layer 2, and the feces sample Directly enter the homogeneous filter bag from the channel formed by the tear 4; The middle part of the filter...

Embodiment 3

[0080] A method for isolating intestinal flora from human feces, which specifically follows the steps below:

[0081] 1) Aseptic sampling

[0082] Collect feces directly in sterilized plastic bags or boxes;

[0083] 2) Aseptic sample addition

[0084] After the discharge port of the plastic bag or plastic box is sterilized with disinfectant, open the homogeneous filter bag, and pour feces into the homogeneous filter bag; the structure of the homogeneous filter bag is the same as that of the homogeneous filter bag in Example 1. 7 is similar, and its difference is only that the filter membrane pore size on its filter membrane interlayer 8 is 0.20mm in this example;

[0085] 3) weighing

[0086] Weigh the total weight of the homogeneous filter bag, and after deducting the self-weight of the pre-weighed homogeneous filter bag, it is the weight of the collected feces sample to obtain 100 grams of feces;

[0087] 4) Add diluent

[0088] Add 300ml of sterile water and seal the h...

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Abstract

The invention relates to a method for separating intestinal flora from human faeces. The method sequentially comprises the following steps: (1) carrying out sampling; (2) carrying out homogenizing and filtering; and (3) carrying out centrifugal separation and washing, specifically, first, carrying out washing by adopting a diluent, carrying out centrifugalizing at the rotation speed of 7000-15000 rpm, wherein the time for each time of centrifugalizing is 5-25 min, and 1-5 times of washing and centrifugalizing are carried out. With the adoption of the method provided by the invention, the pollution problem generated in the preparation process of intestinal flora liquid can be reduced, the discomfort caused to work staff during the preparation process is avoided, and the product has the excellent quality.

Description

technical field [0001] The invention relates to the technical field of microorganism separation and extraction, in particular to a method for isolating intestinal flora from human feces. Background technique [0002] In recent years, due to the general increase of intestinal flora imbalance caused by the abuse of antibiotics, the difficulty of curing intestinal diseases, the cost of research and development of new antibiotics and the increase of new drug-resistant strains and other side effects, researchers once again set their sights on the use of extracts The intestinal flora of healthy people can be used to rebuild the intestinal tract and other diseases caused by the imbalance of intestinal flora. At present, researchers are focusing on the types and curative effects of intestinal flora in clinical treatment of diseases, but for the preparation of intestinal flora, they often use crude methods, for example, first use ordinary plastic boxes, plastic Sampling in bags, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 曾令聪胡犁涛
Owner CHONGQING BEIZHITANG BIOTECH
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