Application of Brazil green propolis in preparation of PDT antitumor synergistic drugs

A synergistic and anti-tumor technology, applied in the field of medicine, can solve the problems of weakening the curative effect, damaging tumor cells, and increasing the level.

Inactive Publication Date: 2017-07-04
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] While PDT damages tumor cells, it will cause an inflammatory response, and the levels of NF-κB and cyclooxygenase-2 (COX-2) will increase, which will make tumor cells evade treatment and promote tumor cell survival. , weaken the efficacy, leading to cancer recurrence

Method used

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  • Application of Brazil green propolis in preparation of PDT antitumor synergistic drugs
  • Application of Brazil green propolis in preparation of PDT antitumor synergistic drugs
  • Application of Brazil green propolis in preparation of PDT antitumor synergistic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Experimental purpose: through the cytotoxicity test and the calculation of the synergy index to show that the BGP combined with PDT method has a synergistic effect on anti-tumor.

[0024] experimental design:

[0025] On the human skin squamous cell carcinoma cell line A431, the MTT method was used to determine the cytotoxicity of different doses of BGP, PpIX-PDT and BGP combined with PpIX-PDT. The results are shown in Table 1.

[0026]

[0027] Table 1

[0028] Result analysis: When BGP 62.5μg / ml was administered alone, the cell survival rate was 73.0±2.0%, and when the concentration reached 75μg / ml, the cell survival rate was only 62.1±2.2%. When PpIX 1.8μM, 2.7μM or 4.0μM was administered alone, the cell survival rate was 74.9±0.9%, 65.9±1.8% and 34.1±2.3%, respectively. When BGP was used in combination with PpIX-PDT, the survival percentage of A431 cells was significantly lower than that of BGP alone group and PpIX-PDT alone group.

[0029] To detect the synergistic effect...

Embodiment 2

[0034] Experimental purpose: To demonstrate that BGP combined with PDT has a synergistic effect on anti-tumor by measuring the expression levels of apoptosis and anti-apoptotic proteins and pro-apoptotic proteins.

[0035] Experimental design: qualitatively and quantitatively determined cell apoptosis caused by BGP, PpIX-PDT and BGP combined with PpIX-PDT on A431 cells by Annexin V-FITC / PI staining. The expression of anti-apoptotic protein Bcl-xL and pro-apoptotic protein Bax, NOXA, and cleaved caspase-3 were detected by Western Blot method.

[0036] (1) After A431 cells are treated with BGP (75μg / ml), PpIX (2.7μM) or BGP combined with PpIX-PDT, the ratio of live cells, early apoptotic cells, late apoptotic cells and necrotic cells. Each group received laser irradiation with an intensity of 27mW / cm2. The result is figure 1 with figure 2 Shown.

[0037] Result analysis: At the experimental dose, almost all groups did not cause necrosis of A431 cells, and apoptosis was the main form...

Embodiment 3

[0041] Experimental purpose: To demonstrate that BGP combined with PDT can reduce cell inflammation by measuring the protein expression level of NF-κB / COX-2 signaling pathway.

[0042] experimental design:

[0043] Western Blot method was used to detect the expression of BGP, PpIX-PDT and BGP combined with PpIX-PDT on the protein expression of NF-κB / COX-2 signaling pathway in A431 cells. .

[0044] Western Blot method was used to determine the changes in the expression levels of inflammation-related proteins such as p-IKKα / β, NF-κB, COX-2, etc. after A431 cells were treated with BGP (75μg / ml), PpIX (2.7μM) and BGP combined with PpIX-PDT . Use β-Actin as internal control. Each group received laser irradiation with an intensity of 27mW / cm2. The result is Figure 4 Shown.

[0045] Result analysis: When only PDT was given, p-IKKα / β in A431 cells increased significantly, the expression of NF-κB was up-regulated, and the protein level of its downstream effect target COX-2 also increased...

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Abstract

The invention discloses an application of Brazil green propolis in the preparation of PDT antitumor synergistic drugs. In PDT antitumor treatment, the ratio of used photo sensitizer to Brazil green propolis is 1:27-75. The cell experiment results show that the cooperation of BGP (Brazil green propolis) and PDT can alleviate cell inflammation and promote the death of tumor cells. The animal experiment results show that cooperation of BGP and PDT can inhibit the tumorigenicity of tumor cells.

Description

Technical field [0001] The invention relates to an application of Brazilian green propolis in the preparation of PDT anti-tumor synergistic drugs, and belongs to the technical field of medicine. Background technique [0002] Tumor is a major disease threatening human health and social development, and its incidence and treatment have always been research hotspots in the field of medicine. The combined application of different tumor treatment methods is an effective strategy to improve the anti-tumor effect and reduce side effects. [0003] Propolis is a fragrant gel-like solid formed by honeybees collecting resin from plant spores or tree trunks, mixing with the secretions of the mandibular glands and wax glands, and processing them. It has a wide range of biological activities. Due to different geographical environments, the active ingredients and pharmacological effects of propolis from different producing areas are also different. In Brazil, which has abundant vegetation and d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K35/644A61P35/00
CPCA61K35/644A61K41/0071A61K2300/00
Inventor 吴亮何玲王呈呈王雨轩汪小燕胡蝶陈星光廖有为牟畅崔雪婷李成婧俞婧婷丁赣成张陆勇
Owner CHINA PHARM UNIV
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