Application of magnolol in preparation of an antitumor drug sensitizer

An anti-tumor drug, the technology of magnolol, which is applied in the field of cell biology and medicine, can solve the problems of weak anti-autooxidation ability and no anti-oxidation activity, so as to increase the sensitivity of tumor multidrug-resistant cells to anti-tumor drugs Sexuality and anti-tumor effect

Inactive Publication Date: 2017-07-07
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The results show that magnolol has weak anti-autooxidation ability in soybean oil and lard; in 10% emulsion system, magnolol has almost no antioxidant activity (Journal of Chinese Cereals and Oils, March 2007, No. 22 No. 2, 71-74)

Method used

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  • Application of magnolol in preparation of an antitumor drug sensitizer
  • Application of magnolol in preparation of an antitumor drug sensitizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062]

[0063] Experimental results:

[0064] KB / VCR and MCF-7 / ADR are two commonly used multidrug-resistant cell lines, and in this example, these two kinds of cells also exhibit the characteristic of multidrug resistance. As shown in Table 1, compared with KB cells, the multiples of drug resistance of KB / VCR cells to VCR and ADR were 81.9 times and 94.4 times, respectively, while MCF-7 / ADR cells were more resistant to VCR and ADR than MCF-7 cells. The drug multiples were 38.1 times and 20.9 times respectively, showing that the drug-resistant cells used in the experiment had multidrug resistance, and the MDR activity was similar to the results reported in the literature.

[0065] The half maximal inhibitory concentration IC of magnolol on the parental cell lines KB and MCF-7 50 76.8 μM and 121.9 μM, respectively, to the IC of drug-resistant cell lines KB / VCR and MCF-7 / ADR 50 Respectively 139.8 μ M and 144.1 μ M, no significant difference between the two, multidrug resis...

Embodiment 2

[0067] Example 2: CCK-8 method detects the reversal effect of magnolol on tumor cell multidrug resistance activity

[0068] KB / VCR, MCF-7 / ADR and HCT-8 / VCR cells were inoculated into 96-well plates at a density of 3500 / well, and after 24 h, different concentrations of VCR, as well as different concentrations of VCR and honokiol were used together with 10 α-MEM with % fetal bovine serum was added to each well. After culturing for 48 h, the culture medium was discarded, and the CCK-8 kit was used to measure the activity of drug-resistant cell lines and their parental cells on VCR and VCR + magnolol in the same manner as in Example 1, and a curve was drawn. Three replicate wells were set for each concentration, and the experiment was repeated 3 times.

[0069] Experimental results:

[0070] The multiplicity of drug resistance of KB / VCR cells to VCR was 81.9 times, and that of MCF-7 / ADR cells to ADR was 20.9 times. When 5M magnolol was added, the sensitivity of KB / VCR cells to ...

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Abstract

The invention belongs to the fields of cell biology and medicine, and relates to the application of magnolol in the preparation of antitumor drug sensitizers. Magnolol is a natural product, which has the effect of reversing the multidrug resistance of tumor cells, and can be used as a reversal agent for tumor multidrug resistance; magnolol also has the effect of increasing the sensitivity of tumor multidrug resistant cells to antitumor drugs, which can Used as a chemosensitizer. The invention also provides a method for inhibiting tumor multidrug-resistant cell proliferation with the pharmaceutical composition combined with antineoplastic drugs and magnolol. The small-molecule compound magnolol in the present invention is developed as a new antitumor drug or its auxiliary component, has obvious tumor suppressing effect, is environmentally friendly, and will provide a new way and means for treating and curing tumors.

Description

technical field [0001] The invention belongs to the field of cell biology and medicine, in particular, the invention relates to a new application of magnolol. The invention also relates to the corresponding pharmaceutical composition and its application method. Background technique [0002] Tumor is an abnormal lesion formed by the body under the action of various carcinogenic factors when a certain cell in a local tissue loses its normal regulation of its growth at the gene level, resulting in its clonal abnormal proliferation. The number of cancer cases in my country is quite large, accounting for 55% of the total number of cases in the world. [0003] Benign tumors have less impact on the body, mainly showing symptoms of local compression and obstruction. Malignant tumors have a serious impact on the body due to their immature differentiation, rapid growth, infiltration and damage to the structure and function of organs, and the possibility of metastasis. In addition t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/05A61K45/06A61P35/00
CPCA61K31/05A61K45/06
Inventor 余龙朱恒锐
Owner FUDAN UNIV
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