Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for assaying T lymphocytes subsets of chicken thymuses by flow cytometry

A flow cytometry and lymphocyte technology, which is applied in the field of flow cytometry detection of chicken thymus T lymphocyte subsets, achieves the effects of improving authenticity and objectivity, broad application prospects and simple operation steps.

Inactive Publication Date: 2017-07-14
CHINA WEST NORMAL UNIVERSITY
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the present invention aims at the problems of target cell selection, compensation adjustment and parameter selection in the process of sample processing, detection and analysis, and provides a method for detecting chicken thymus T lymphocyte subsets by flow cytometry, aiming to establish a A Stable and Standardized Detection Method of Chicken Thymus T Lymphocyte Subpopulation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for assaying T lymphocytes subsets of chicken thymuses by flow cytometry
  • Method for assaying T lymphocytes subsets of chicken thymuses by flow cytometry
  • Method for assaying T lymphocytes subsets of chicken thymuses by flow cytometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0052] Step 1, after killing the test chicken, obtain thymus tissue and prepare single cell suspension

[0053] A single intraperitoneal injection of cyclophosphamide was given to chickens at a certain dose level to establish an immunosuppressive model. The successfully modeled chickens were sacrificed by dislocation of the cervical vertebrae and killed immediately, the thymus tissue was removed, the blood stains were blotted with filter paper, the surrounding connective tissue was trimmed with ophthalmic scissors, and stored in PBS at 4°C;

[0054] Take the preserved sample, put it in a watch glass, cut it repeatedly with ophthalmic scissors until the tissue becomes muddy, add an appropriate amount of PBS solution, and mix well; then filter it with a 300-mesh nylon mesh to obtain the filtrate; at a speed of 600-1000r / min Centrifuge the filtrate for 5 minutes, discard the supernatant; then add 1mL PBS solution, vortex to mix, centrifuge again, discard the supernatant; resuspen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for assaying T lymphocytes subsets of chicken thymuses by flow cytometry, which includes the following steps: after a chicken is killed, thymus tissues are obtained, and single-cell suspension is prepared; the single-cell suspension is added with 1 dose of anti-chicken CD3 monoclonal antibody, 1 dose of anti-chicken CD4 monoclonal antibody and 1 dose of anti-chicken CD8 monoclonal antibody, vortex mixing is carried out, and staining is carried out under 4 DEG C away from light; stained cell solution is washed with PBS and cells are resuspended, and a flow cytometer is used for assaying; an assay result is analyzed, and thereby a proportion of T lymphocytes subsets of chicken thymuses is obtained. For the issues of target cell choice, compensative regulation and parameter selection in the process of sample processing, assay and analysis, the method for assaying T lymphocytes subsets of chicken thymuses by flow cytometry disclosed by the invention establishes a stable and standard method for assaying T lymphocytes subsets of chicken thymuses.

Description

technical field [0001] The invention belongs to the technical field of cell detection, in particular to a method for detecting chicken thymus T lymphocyte subsets by flow cytometry. Background technique [0002] Flow cytometry (Flow Cytometry, FCM) is a technique that uses flow cytometry for quantitative analysis and sorting of single cells. Its working principle is to perform multi-parameter quantitative analysis after labeling or capturing single cells or molecules with fluorescently labeled monoclonal antibodies or fluorescent microspheres. During the detection process, the information related to the cell size and intracellular substance density can be obtained respectively through the non-fluorescent scattering signal, that is, the forward scattered light FSC and the side scattered light SSC signal, so that the cells can be grouped. The processing can form the basis for further analysis of the characteristics of specific target cell populations. [0003] The biggest ad...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14
CPCG01N15/14G01N2015/1006
Inventor 彭西张泽钧袁施彬吴邦元
Owner CHINA WEST NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com