Method for extracting chitin from fruit fly maggots
A technology for chitin and fly maggots is applied in the field of extracting and separating effective components, which can solve the problems of poor environmental conditions, demanding process conditions and high extraction costs, and achieve the effects of protecting the environment, improving hydrolysis efficiency and avoiding ring damage.
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Embodiment 1
[0025] A method for extracting chitin from fruit fly maggots includes the following steps:
[0026] (1) After disinfecting and cleaning the fresh fruit fly larvae, squeeze it with a pressing roller to separate the larva skin from the visceral mass slurry. The larva skin is used as the raw material for extracting chitin, and the larva skin is washed and drained. ,Place in a microwave oven, control the temperature at 75℃, and heat for 15min;
[0027] (2) The dried fly maggot skin obtained in step (1) is placed in a pulverizer for pulverization to obtain fly maggot powder. The fly maggot powder is added to a certain volume of water and stirred to obtain fly maggot slurry, and then the slurry is placed in power The compound protease is added to the 400W ultrasonic device for hydrolysis at a temperature of 55°C for a certain period of time; wherein the mass-volume ratio (g / mL) of the fly maggot meal to water is 1:15; the compound protease is trypsin, Papain and alkaline protease are co...
Embodiment 2
[0031] A method for extracting chitin from fruit fly maggots includes the following steps:
[0032] (1) After disinfecting and cleaning the fresh fruit fly larvae, squeeze it with a pressing roller to separate the larva skin from the visceral mass slurry. The larva skin is used as the raw material for extracting chitin, and the larva skin is washed and drained. ,Place in a microwave oven, control the temperature at 70℃, and heat for 20min;
[0033] (2) The dried fly maggot skin obtained in step (1) is placed in a pulverizer for pulverization to obtain fly maggot powder. The fly maggot powder is added to a certain volume of water and stirred to obtain fly maggot slurry, and then the slurry is placed in power In a 500W ultrasonic device at a temperature of 50°C, a compound protease is added for hydrolysis for a certain period of time; wherein the mass-volume ratio (g / mL) of the fly maggot powder and water is 1:20; the compound protease is trypsin, Papain and alkaline protease are co...
Embodiment 3
[0037] A method for extracting chitin from fruit fly maggots includes the following steps:
[0038] (1) After disinfecting and cleaning the fresh fruit fly larvae, squeeze it with a pressing roller to separate the larva skin from the visceral mass slurry. The larva skin is used as the raw material for extracting chitin, and the larva skin is washed and drained. ,Place in a microwave oven, control the temperature at 80℃, and heat for 10min;
[0039] (2) The dried fly maggot skin obtained in step (1) is placed in a pulverizer for pulverization to obtain fly maggot powder. The fly maggot powder is added to a certain volume of water and stirred to obtain fly maggot slurry, and then the slurry is placed in power In a 300W ultrasonic device at a temperature of 60°C, a compound protease is added for hydrolysis for a certain period of time; wherein the mass-volume ratio (g / mL) of the fly maggot powder and water is 1:10; the compound protease is trypsin, Papain and alkaline protease are co...
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