Primers and kit for detecting tomato gray leaf spot pathogenic bacteria
The technology of a pathogenic bacteria and a kit is applied in the field of primers and kits for detecting tomato gray leaf spot pathogen, which can solve the problems of tomato production reduction and economic loss of growers, and achieve the effects of strong primer specificity and high detection accuracy
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Embodiment 1
[0021] According to the conserved sequence of glyceraldehyde-3-phosphate dehydrogenase (gpd) in GenBank (GenBank No. .1, AF443886.1, KF479194.1), design primers Stem-F and Stem-R, the amplification length is 489bp, clone, sequence and analyze the fragments. The specific primer sequence of tomato ash leaf spot pathogen is as follows:
[0022] Upstream primer (Stem-F): 5'-CTGAACCCACCGCTTTTGCCATGTC-3' (SEQ ID NO: 1 in the sequence listing);
[0023] Downstream primer (Stem-R): 5'-CGAACATGGGAGCGTCGGCGGAA-3' (SEQ ID NO: 2 in the sequence listing).
[0024] SEQ ID NO: 1 in the sequence listing consists of 25 bases, SEQ ID NO: 2 consists of 23 bases, and the length of the amplified fragment is 489bp.
Embodiment 2
[0026] A kit for detecting the pathogen of tomato gray leaf spot, the kit includes a box body and 4 PCR tubes, and 2×Taq PCR MasterMix and ddH are respectively installed in the 2 PCR tubes 2 O: The other two PCR tubes are equipped with upstream primers for detecting the pathogen of tomato gray leaf spot and downstream primers for detecting the pathogen of tomato gray leaf spot respectively.
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