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Primers and kit for detecting tomato gray leaf spot pathogenic bacteria

The technology of a pathogenic bacteria and a kit is applied in the field of primers and kits for detecting tomato gray leaf spot pathogen, which can solve the problems of tomato production reduction and economic loss of growers, and achieve the effects of strong primer specificity and high detection accuracy

Inactive Publication Date: 2017-07-25
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, tomato gray leaf spot disease has shown a trend of more serious outbreaks. Misdiagnosis or untimely control often leads to a reduction in tomato production, generally by about 20%, and some even by more than 80%, causing serious economic losses to growers. Loss (Cao Shoujun et al., 2011; Liu Guohua, 2012)

Method used

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  • Primers and kit for detecting tomato gray leaf spot pathogenic bacteria
  • Primers and kit for detecting tomato gray leaf spot pathogenic bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0021] According to the conserved sequence of glyceraldehyde-3-phosphate dehydrogenase (gpd) in GenBank (GenBank No. .1, AF443886.1, KF479194.1), design primers Stem-F and Stem-R, the amplification length is 489bp, clone, sequence and analyze the fragments. The specific primer sequence of tomato ash leaf spot pathogen is as follows:

[0022] Upstream primer (Stem-F): 5'-CTGAACCCACCGCTTTTGCCATGTC-3' (SEQ ID NO: 1 in the sequence listing);

[0023] Downstream primer (Stem-R): 5'-CGAACATGGGAGCGTCGGCGGAA-3' (SEQ ID NO: 2 in the sequence listing).

[0024] SEQ ID NO: 1 in the sequence listing consists of 25 bases, SEQ ID NO: 2 consists of 23 bases, and the length of the amplified fragment is 489bp.

Embodiment 2

[0026] A kit for detecting the pathogen of tomato gray leaf spot, the kit includes a box body and 4 PCR tubes, and 2×Taq PCR MasterMix and ddH are respectively installed in the 2 PCR tubes 2 O: The other two PCR tubes are equipped with upstream primers for detecting the pathogen of tomato gray leaf spot and downstream primers for detecting the pathogen of tomato gray leaf spot respectively.

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Abstract

The invention discloses primers and a kit for detecting tomato gray leaf spot pathogenic bacteria. The primers include an upstream primer (see SEQ ID NO:1 for sequence) and a downstream primer (see SEQ ID NO:2 for sequence). The primers and kit can quickly and accurately identify tomato gray leaf spot pathogenic bacteria, and are of great significance for early detection and disease control of tomato gray leaf spots.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer and a kit for detecting tomato gray leaf spot pathogen. Background technique [0002] Tomato (Solanum esculentum L.) is one of the most important vegetables in my country. It is deeply loved by growers and consumers. The planting area continues to expand, and now it can be planted every year. The factors restricting tomato production are difficult to predict the market conditions, and the most important ones are the many diseases and insect pests and the serious damage. Among them, tomato gray leaf spot disease (Tomato gray leaf spot disease, GLSD) is a worldwide disease. In recent years, tomato gray leaf spot disease has shown a trend of more serious outbreaks. Misdiagnosis or untimely control often leads to a reduction in tomato production, generally by about 20%, and some even by more than 80%, causing serious economic losses to growers. loss (Cao Shoujun et al., 2011; ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
CPCC12Q1/6895
Inventor 叶青静周国治王荣青阮美颖杨悦俭姚祝平李志邈万红建程远
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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