Multiplex RT-PCR Detection Method for Latent Viruses and Viroids in Apple

An apple latent virus, RT-PCR technology, applied in microorganism-based methods, biochemical equipment and methods, and microbial determination/inspection, etc., can solve the problems of high efficiency of multiplex PCR, no simultaneous detection, etc., to improve the results. The effect of discriminating accuracy, reducing non-specific amplification, and improving detection accuracy and sensitivity
CN104164517BInactive Publication Date: 2016-06-22CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2016-06-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a multiple RT-PCR detection method, which can simultaneously detect whether leaves, twigs, flowers or fruits of an apple tree bring three latent viruses namely apple chlorotic leaf spot virus, apple stem grooving virus, and apple stem pitting virus, and three apple scar skin viroids namely apple scar skin viroid, apple dimple fruit viroid, and apple fruit crinkle viroid or not. The detection method has the advantages of clear detection result, convenient operation, rapid reaction, and high sensitivity, and overcomes the shortages of time consuming, tedious steps, difficulty in distinguishing the stripes, and low sensitivity in the prior art. The invention provides a rapid detection method, which is easy for promotion and application, for detecting apple viruses.
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Description

technical field

[0001] The invention relates to the field of plant protection and molecular biology detection, in particular to a multiple RT-PCR detection method for apple latent virus and viroid. Background technique

[0002] In the process of apple cultivation and production, virus disease has always been one of the main factors that seriously affect the yield and quality of fruit. After the apple tree is infected by the virus, it will be poisoned for life. The virus proliferates in the tree, interferes and destroys the normal physiological function of the tree, resulting in decreased growth, decreased yield, poor quality, and intolerance to storage, which has caused the production of apples in my country. serious economic losses. At present, the viruses that have been discovered and identified in my country mainly include: apple chloroticleafspot virus (ACLSV), apple stem grooving virus (ASGV), apple stem pox virus (Applestempitting virus, ASPV), apple rust virus (Apples...

Claims

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