A kind of dna extraction method of horny layer of animal horn medicinal material
An extraction method and animal horn technology are applied in the field of identification of traditional Chinese medicinal materials, and can solve the problems of inability to perform PCR on horn medicinal materials, incomplete keratinocyte lysis, low DNA template concentration, etc., and achieve the effects of improving the incomplete lysis and the lysis effect.
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[0016] Example 1: Collect all horn animal medicinal materials and folk collection samples (including horns and horns) in Hebei Anguo medicinal materials market and Anhui Bozhou medicinal materials market, and study the DNA extraction method of horn medicinal materials (cuticle), so that the extracted The DNA quality can meet the requirements of conventional PCR reactions.
[0017] 1. Materials and Instruments
[0018] The collected sample information is shown in Table 1. For sample photos, see figure 1 . only by virtue of figure 1 In the photo, the species source information of the sample cannot be identified by traditional identification methods, and for samples with a small amount of DNA template, it is difficult to meet the requirements of PCR sequencing by conventional DNA extraction, so it is also impossible to identify the species source of the sample. Observation is easy to confuse the sample species, and misjudgment and misjudgment occur, which eventually lead to m...
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[0047] Experimental example: using the old croissant horn sample that has been preserved for more than 10 years, it is processed according to the conventional kit treatment method and the method of the present invention respectively. The conventional kit treatment method does not include the (3) step, and the method of the present invention includes the (3) step. Blood / cell / tissue genomic DNA extraction kit (spin column type) from Tiangen Biochemical Technology Co., Ltd., catalog number: DP304 (including Proteinase K, buffer GA, buffer GB, buffer GD, washing solution PW, adsorption column CB3). See Table 6 for sample information.
[0048] Table 6: Information on samples stored for more than 10 years
[0049] Numbering sample name Sample storage time place of origin 1 Antelope Horns (Size) 15 years siberia 2 Antelope horn block 13 years Russia 3 Tibetan antelope horns (height) 12 years Xinjiang
[0050] The specific steps are:...
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