The invention belongs to the technical field of microalga biology and particularly discloses a method for culturing chlorella vulgaris. The method comprises the steps of culture vessel disinfecting, nutrient solution preparing, inoculation, culture, harvesting and the like. According to the method, firstly, the chlorella vulgaris is cultured for 2-4 days under most appropriate growth conditions (the intensity of illumination is 3,000Lx-4,500Lx, the water temperature is 25 DEG C to 30 DEG C, and the carbon dioxide recruitment is 2mg / L-3mg / L), thus, the chlorella vulgaris is subjected to mass propagation and growth, and an initial concentration of alga cells in a nutrient solution is increased; then, culture is carried out for 5-10 days at a low temperature (0 DEG C to 5 DEG C), so as to enable the chlorella vulgaris to gradually adapt to a low-temperature growth environment; and finally, the temperature rises to 5 DEG C to 10 DEG C, culture is continued for 1-2 days, so as to enable the chlorella vulgaris to continue to grow, and thus, the chlorella vulgaris can directly serve as an alga source cultured by an outdoor pond (water temperature: 6 DEG C to 9 DEG C) in winter. According to the method, the chlorella vulgaris can grow at low temperatures, a culture mode is simple, the death rate of the chlorella vulgaris can be lowered, and generation of other sundry algae is avoided.