Method for culturing chlorella vulgaris

A cultivation method and technology of chlorella, which are applied in the field of cultivation of chlorella, can solve problems such as cumbersome cultivation methods, aggravated death of chlorella, disordered growth and development of chlorella, etc.

Pending Publication Date: 2021-04-23
曹礼刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the temperature is too high, it is easy to cause the death of some chlorella. The dead chlorella will acidify the water, further aggravate the death of chlorella, and finally cause the appearance of sudden death of chlorella. At the same time, the method takes 24 hours as a cycle, High and low temperature adjustment is required every 8 hours, and 10-14 cycles of cultivation are required. The cultivation method is relatively cumbersome, and the temperature adjustment time is too frequent, which may easily cause disordered growth and development of Chlorella

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A method for cultivating chlorella, comprising the steps of:

[0052] (1) Disinfect the culture container, and then clean the culture container with water.

[0053] (2) prepare nutrient solution, the preparation method of nutrient solution is:

[0054] Component A: 50 mg of ferric chloride, 2.0 mg of manganese chloride, 200 mg of 45% boron oxide, 1 g of potassium dihydrogen phosphate, 200 mg of tetrasodium edetate, 100 mg of sodium carbonate or sodium EDTA.

[0055] Component B: 0.5 grams of zinc chloride, 0.5 grams of cobalt chloride, 0.2 grams of ammonium molybdate, and 0.5 grams of copper sulfate.

[0056] Put component A and component B into 1 ton of fresh water, fully dissolve, then add 2.0 grams each of vitamin B1 and B12, stir evenly, and prepare a culture solution. The culture solution was heated at 90°C for 5 minutes before use.

[0057] (3) Vaccination:

[0058] Select algal species with strong viability and vigorous growth, and inoculate them in the morni...

Embodiment 2

[0069] A method for cultivating chlorella, comprising the steps of:

[0070] (1) Disinfect the culture container, and then clean the culture container with water.

[0071] (2) prepare nutrient solution, the preparation method of nutrient solution is:

[0072] Component A: 45 mg of ferric chloride, 1.8 mg of manganese chloride, 200-210 mg of 45% boron oxide, 1.25 g of potassium dihydrogen phosphate, 210 mg of tetrasodium edetate, 310 mg of sodium carbonate or sodium EDTA 110 mg.

[0073] Component B: 0.6 grams of zinc chloride, 0.6 grams of cobalt chloride, 0.25 grams of ammonium molybdate, and 0.6 grams of copper sulfate.

[0074] Component C: 2 grams of chlorella powder. The preparation method of the chlorella powder is as follows: drying the chlorella at a temperature of 60 DEG C, the water content of the dried chlorella is lower than 5%, and then pulverizing it into a 200-mesh powder. Put A component, B component and C component into 1 ton of fresh water, fully dissolve...

Embodiment 3

[0088] A method for cultivating chlorella, comprising the steps of:

[0089] (1) Disinfect the culture container, and then clean the culture container with water.

[0090] (2) prepare nutrient solution, the preparation method of nutrient solution is:

[0091] Component A: 48 mg of ferric chloride, 2.0 mg of manganese chloride, 200 mg of 45% boron oxide, 1 g of potassium dihydrogen phosphate, 200 mg of tetrasodium edetate, 300 mg of sodium carbonate or 100 mg of sodium EDTA ;

[0092] Component B: 0.5 grams of zinc chloride, 0.5 grams of cobalt chloride, 0.2 grams of ammonium molybdate, and 0.5 grams of copper sulfate;

[0093] Component C: 5 grams of chlorella powder. The preparation method of the chlorella powder is as follows: the chlorella is dried at a temperature of 50 DEG C, and the water content of the dried chlorella is lower than 5%, and then crushed into a 300-mesh powder.

[0094]Put A component, B component and C component into 1 ton of fresh water, fully disso...

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Abstract

The invention belongs to the technical field of microalga biology and particularly discloses a method for culturing chlorella vulgaris. The method comprises the steps of culture vessel disinfecting, nutrient solution preparing, inoculation, culture, harvesting and the like. According to the method, firstly, the chlorella vulgaris is cultured for 2-4 days under most appropriate growth conditions (the intensity of illumination is 3,000Lx-4,500Lx, the water temperature is 25 DEG C to 30 DEG C, and the carbon dioxide recruitment is 2mg / L-3mg / L), thus, the chlorella vulgaris is subjected to mass propagation and growth, and an initial concentration of alga cells in a nutrient solution is increased; then, culture is carried out for 5-10 days at a low temperature (0 DEG C to 5 DEG C), so as to enable the chlorella vulgaris to gradually adapt to a low-temperature growth environment; and finally, the temperature rises to 5 DEG C to 10 DEG C, culture is continued for 1-2 days, so as to enable the chlorella vulgaris to continue to grow, and thus, the chlorella vulgaris can directly serve as an alga source cultured by an outdoor pond (water temperature: 6 DEG C to 9 DEG C) in winter. According to the method, the chlorella vulgaris can grow at low temperatures, a culture mode is simple, the death rate of the chlorella vulgaris can be lowered, and generation of other sundry algae is avoided.

Description

technical field [0001] The invention relates to the field of microalgae biology, in particular to a method for cultivating chlorella. Background technique [0002] Chlorella is a single-celled plant with many types, most of which live in fresh water, and a few live in oceans. According to botanical classification, Chlorella belongs to the genus Chlorella of the Chlorophyta Chlorophyta Chlorophyceae Chlorophyceae Oocystaceae Chlorella. It is small in size and generally 3-8 μm in diameter. Under a microscope, it needs to be magnified 400-600 times. above to see. Chlorella contains very high nutritional components, with a protein content of 50% to 60% (5 times that of eggs), a fat content of 10% to 30%, and a variety of vitamins, minerals, nucleic acids and chlorophyll. Chlorella's biologically active substances, glycoproteins and polysaccharides, as well as up to 13% of nucleic acid and other substances, have the functions of enhancing human immunity, anti-cancer, lowering b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/38C12R1/89
CPCC12N1/12C12N1/38
Inventor 曹礼刚
Owner 曹礼刚
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