Method for culturing chlorella vulgaris
A cultivation method and technology of chlorella, which are applied in the field of cultivation of chlorella, can solve problems such as cumbersome cultivation methods, aggravated death of chlorella, disordered growth and development of chlorella, etc.
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Embodiment 1
[0051] A method for cultivating chlorella, comprising the steps of:
[0052] (1) Disinfect the culture container, and then clean the culture container with water.
[0053] (2) prepare nutrient solution, the preparation method of nutrient solution is:
[0054] Component A: 50 mg of ferric chloride, 2.0 mg of manganese chloride, 200 mg of 45% boron oxide, 1 g of potassium dihydrogen phosphate, 200 mg of tetrasodium edetate, 100 mg of sodium carbonate or sodium EDTA.
[0055] Component B: 0.5 grams of zinc chloride, 0.5 grams of cobalt chloride, 0.2 grams of ammonium molybdate, and 0.5 grams of copper sulfate.
[0056] Put component A and component B into 1 ton of fresh water, fully dissolve, then add 2.0 grams each of vitamin B1 and B12, stir evenly, and prepare a culture solution. The culture solution was heated at 90°C for 5 minutes before use.
[0057] (3) Vaccination:
[0058] Select algal species with strong viability and vigorous growth, and inoculate them in the morni...
Embodiment 2
[0069] A method for cultivating chlorella, comprising the steps of:
[0070] (1) Disinfect the culture container, and then clean the culture container with water.
[0071] (2) prepare nutrient solution, the preparation method of nutrient solution is:
[0072] Component A: 45 mg of ferric chloride, 1.8 mg of manganese chloride, 200-210 mg of 45% boron oxide, 1.25 g of potassium dihydrogen phosphate, 210 mg of tetrasodium edetate, 310 mg of sodium carbonate or sodium EDTA 110 mg.
[0073] Component B: 0.6 grams of zinc chloride, 0.6 grams of cobalt chloride, 0.25 grams of ammonium molybdate, and 0.6 grams of copper sulfate.
[0074] Component C: 2 grams of chlorella powder. The preparation method of the chlorella powder is as follows: drying the chlorella at a temperature of 60 DEG C, the water content of the dried chlorella is lower than 5%, and then pulverizing it into a 200-mesh powder. Put A component, B component and C component into 1 ton of fresh water, fully dissolve...
Embodiment 3
[0088] A method for cultivating chlorella, comprising the steps of:
[0089] (1) Disinfect the culture container, and then clean the culture container with water.
[0090] (2) prepare nutrient solution, the preparation method of nutrient solution is:
[0091] Component A: 48 mg of ferric chloride, 2.0 mg of manganese chloride, 200 mg of 45% boron oxide, 1 g of potassium dihydrogen phosphate, 200 mg of tetrasodium edetate, 300 mg of sodium carbonate or 100 mg of sodium EDTA ;
[0092] Component B: 0.5 grams of zinc chloride, 0.5 grams of cobalt chloride, 0.2 grams of ammonium molybdate, and 0.5 grams of copper sulfate;
[0093] Component C: 5 grams of chlorella powder. The preparation method of the chlorella powder is as follows: the chlorella is dried at a temperature of 50 DEG C, and the water content of the dried chlorella is lower than 5%, and then crushed into a 300-mesh powder.
[0094]Put A component, B component and C component into 1 ton of fresh water, fully disso...
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