A screening method for high-efficiency pollutant-degrading bacteria based on microplate culture

Abstract

Belonging to the technical field of environmental governance, the invention particularly relates to a method of refractory pollutant effective bacterium culture by a 96-pore microwell plate, thus realizing high throughput and rapid screening of strains. The method comprises: culturing a lot of single strains isolated from a strain mixture on the microwell plate, and judging the metabolic process and pollutant degradation ability of the strains by dye (MTT or XTT) developing, thus screening out strains with a strong pollutant degradation ability. Compared with the traditional strain screening methods based on shake flask culture, the method has the outstanding advantages of less agent (including microbial inoculums) consumption, short culture period, simple equipment, small experimental space occupation, and small experimental workload, etc. By applying the method, high throughput rapid detection of mass strains can be realized, the strain screening time can be substantially shortened, and the strain screening efficiency is significantly increased.

Description

technical field [0001] The invention belongs to the field of environmental pollution control and treatment, and in particular relates to a method for screening highly efficient pollutant-degrading bacteria based on microplate culture. Background technique [0002] In the work of environmental pollution control, the environmental biological treatment technology that uses microorganisms to degrade pollutants has always been the mainstream technology for pollutant treatment. The targets of environmental biotechnology are generally various organic pollutants or inorganic pollutants with reducing properties, and the ability of microorganisms to degrade these pollutants has a decisive impact on the treatment effect. When the treatment object contains pollutants that are difficult to biodegrade (for example, for some industrial wastewater and waste gas), screening and application of highly efficient pollutant-degrading bacteria (called bioaugmentation) often have obvious effects an...

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings of the above-mentioned prior art, based on the characteristics of microplates and the requirements for screening highly efficient degrading bacteria of refractory pollutants, the present invention provides a method for screening highly efficient degrading bacteria of pollutants based on microplate culture. A large number of single strains isolated from the strain mixture are cultured on the same microplate at the same time, and the metabolism and degradation ability of the strains are judged by MTT (thiazolium blue) color development; the agents (including bacterial agents) using this method Less consumption, short culture cycle, no need to measure the concentration of pollutants, small space occupation, greatly reduced workload of experimenters, can realize simultaneous detection of a large number of strains at the same time, significantly shorten the time for strain screening, and improve the efficiency of strain screening ; Invented to replace the concentration analysis of pollutants with the method of color development, so as to realize the rapid and efficient screening of pollutant-degrading bacteria