Ultrasonic extraction method of Acanthopanax giraldii polysaccharide

The technology of the polysaccharide and extraction method is applied in the field of ultrasonic extraction of the polysaccharide of Rhizoma Rhizoma Rhizoma Rhizoma, and can solve the problems of less research on the polysaccharide extraction process of Rhizoma Rhizoma Rhizoma Rhizoma, only 2.5%, low extraction efficiency, etc., and achieves improvement. The effect of protein removal, low extraction temperature and improved extraction rate

Active Publication Date: 2014-04-16
WEIFANG MEDICAL UNIV
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Problems solved by technology

[0003] Although great progress has been made in the research on the pharmacological effects of Acanthopanax polysaccharides, there are few studies on the extraction process of Acanthopanax polysaccharides, and most of them stay in the traditional boiling water extraction method.
The patent "Acanthus radix polysaccharides, preparation process, application and composition of the compound polysaccharides" and the articles "Experimental Research on Separation and Purification of Acanthopanax polysaccharides" and "Determination of Polysaccharide Content and Acute Toxicity Experimental Research of radix radicis" were published The methods for extracting the polysaccharides of Acanthopanax orientalis respectively are all high-temperature boiling water extraction methods, and the extraction efficiency is relatively low. Among them, the extraction efficiency in "Experimental Research on the Separation and Refining of Acanthopanax orientalis Polysaccharides" is 0.39%; Content Determination and Acute Toxicity Experimental Research" mentioned that the extraction rate was only 2.5% after the cumulative extraction time reached 3.5h

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  • Ultrasonic extraction method of Acanthopanax giraldii polysaccharide
  • Ultrasonic extraction method of Acanthopanax giraldii polysaccharide
  • Ultrasonic extraction method of Acanthopanax giraldii polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1: according to the following steps to extract the polysaccharide

[0055] (1) Pulverizing step: after drying the five-five-natured orchids at 80°C to constant weight, pulverize them to 140-150 mesh.

[0056] (2) Ultrasonic extraction step: take 500g of orientalis micropowder, add 11L of water and soak for 5h, the ultrasonic frequency is 30KHz, the ultrasonic power is 500W, the extraction temperature is 65°C, extract for 50min, filter, and collect the filtrate.

[0057] (3) Protein removal step: pressure -0.09MPa, temperature 70°C, concentrate the extract to 1L under reduced pressure, add 50g of trichloroacetic acid, let stand for 10h to precipitate after fully dissolved, filter and collect the filtrate.

[0058] (4) Alcohol precipitation step: add water to 6.16L, dilute the sugar concentration in the solution to 4mg / mL, then add 18.5L of absolute ethanol, stand at 0°C for 24h, and collect the precipitate by filtration.

[0059] (5) Freeze-drying step: redis...

Embodiment 2

[0060] Embodiment 2: According to the following method steps, the polysaccharides of Acanthopanax polysaccharides are extracted

[0061] (1) Pulverization step: pulverize the five-five-scenteda to 135-145 mesh at 70°C.

[0062] (2) Ultrasonic extraction step: take 2000g of orangyana micropowder, add 40L of water and soak for 3 hours, the ultrasonic frequency is 50KHz, the ultrasonic power is 600W, the extraction temperature is 75°C, extract for 40min, filter, and collect the filtrate.

[0063] (3) Protein removal step: the pressure is -0.11MPa, the temperature is 60°C, the extract is concentrated under reduced pressure to 3.4L, 187g of trichloroacetic acid is added, and after being fully dissolved, stand for 12 hours to precipitate, filter and collect the filtrate.

[0064] (4) Alcohol precipitation step: add water to 18.5L, dilute the sugar concentration in the solution to 6mg / mL, then add 75.5L of absolute ethanol, stand at 4°C for 12h, and collect the precipitate by filtrat...

Embodiment 3

[0066] Embodiment 3: according to the following method steps to extract the polysaccharides of Acanthopanax polysaccharides

[0067] (1) Pulverizing step: pulverize the five-five-flowered five-five-scented root at 75°C to 130-140 mesh.

[0068] (2) Ultrasonic extraction step: take 1000g of orientalis micropowder, add 21L of water and soak for 4h, ultrasonic frequency is 40KHz, ultrasonic power is 400W, extraction temperature is 70°C, extract for 45min, filter, and collect the filtrate.

[0069] (3) Protein removal step: pressure -0.10MPa, temperature 65°C, concentrate the extract to 2.1L under reduced pressure, add 126g trichloroacetic acid, wait for 11h to precipitate after fully dissolved, filter and collect the filtrate.

[0070] (4) Alcohol precipitation step: add water to 11.2L, dilute the sugar concentration in the solution to 5mg / mL, then add 39L of absolute ethanol, stand at 2°C for 18h, and collect the precipitate by filtration.

[0071] (5) Freeze-drying step: redis...

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Abstract

The invention provides an ultrasonic extraction method of Acanthopanax giraldii polysaccharide, belonging to the field of preparation of traditional Chinese medicines. The method comprises the following steps: drying Acanthopanax giraldii at 70-80 DEG C, and pulverizing by a pulverizer; immersing Acanthopanax giraldii in water in a weight ratio of 1:(20-22), extracting Acanthopanax giraldii polysaccharide under the auxiliary action of ultrasonic, and filtering to obtain an extract; concentrating the extract under reduced pressure to 1 / 10-1 / 12 of the original volume, adding trichloroacetic acid, sufficiently dissolving, standing for 10-12 hours, and filtering to collect the filtrate; adding ethanol into the filtrate, sufficiently stirring, standing and collecting a precipitate; and redissolving in water, and carrying out freeze-drying to obtain the Acanthopanax giraldii polysaccharide. The traditional method mostly adopts a boiling water extraction process, has the defects of high energy consumption and low extraction efficiency, easily destroys the polysaccharide structure, and influences the biological functions of the polysaccharide. The method can obviously enhance the polysaccharide extraction rate, and has the advantages of shorter extraction time and low energy consumption; and the obtained Acanthopanax giraldii polysaccharide has an obvious action on improving hypoimmunity caused by chemotherapeutic drugs.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine pharmacy, in particular to an ultrasonic extraction method of five-five-scented polysaccharide. Background technique [0002] Acanthopanax giraldii Harms is a plant of the genus Aralis in the family Araliaceae. Its stem bark is used as medicine. It is warm in nature and pungent in taste. Pain, wind-cold-damp arthralgia, weakness of feet and knees, skin rheumatism and impotence and sac dampness, etc. In recent years, pharmacological studies have shown that polysaccharides are one of the main active ingredients of medicines. Lv Xiaoying et al. (Journal of Practical Cancer. 2000.16 (1): 6-8) found that Acanthopanax polysaccharides can inhibit the colony formation of gastric cancer cells and induce apoptosis of human gastric cancer cells. Zhang Lixia et al. (Chinese Medicinal Materials. 1994.17(5): 36-38) showed that the polysaccharides of Acanthopanax rubiosa participate in the humoral i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 陈永梁淑娟
Owner WEIFANG MEDICAL UNIV
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