Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rapid detection method for sampling swab and fully-integrated analysis system for swab sampled sample

An analysis system, fully integrated technology, applied in biochemical equipment and methods, microbial determination/inspection, application, etc., can solve problems such as limiting the application experimental scenarios of nucleic acid detection of new coronavirus infection, affecting the process of solving new coronavirus infection, etc. The effect of reducing manual operation steps and labor intensity, avoiding manual operation errors, and reducing consumption

Active Publication Date: 2021-10-29
TSINGHUA UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This not only seriously affects the solution process of rapid detection and diagnosis of new coronavirus infection health and epidemic prevention that the world is facing today, but also seriously limits the application experimental scenarios of new coronavirus infection nucleic acid detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rapid detection method for sampling swab and fully-integrated analysis system for swab sampled sample
  • Rapid detection method for sampling swab and fully-integrated analysis system for swab sampled sample
  • Rapid detection method for sampling swab and fully-integrated analysis system for swab sampled sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Such as figure 1 As shown, the swab sampling fully integrated analysis system provided in this embodiment includes a preprocessing unit 1 , a mixing unit 2 , a sampling amplification unit 3 , an optical detection unit 4 , a temperature control unit 5 and a motion control unit 6 .

[0077] The preprocessing unit 1 is configured to store the sampling swab, elute the substance to be detected on the sampling swab, and lyse and separate the nucleic acid;

[0078] The mixing unit 2 is configured to uniformly mix the nucleic acid separated by lysing the sampling swab with the reaction reagent;

[0079] The sampling amplification unit 3 is configured to distribute the mixture of samples and reagents to each independent detection unit, so as to realize parallel amplification of multi-channel nucleic acids;

[0080] The optical detection unit 4 is configured to detect the sampling and amplification unit 3 by means of one-time imaging; or to detect the sampling and amplification...

Embodiment 2

[0106] The sampling swab provided in this embodiment is directly put into the rapid detection method, comprising the following steps:

[0107] S1. Put the sampling swab SZ into the preservation tube PC pre-installed with the lysis preservation solution PC4 that inactivates and prevents RNA degradation, tighten the sealing tube cap PC2, and put the preservation tube PC with the sampling swab into the full integration Analyze the system and start it, wherein, the pathogenic bacteria adhered on the sampling swab SZ are inactivated by the lysed preservation solution PC4, and the nucleic acid (DNA, RNA) is fixed without degradation;

[0108] S2. Under the action of the rotating motor M1, through the high-speed rotation of the crushing rod PC3, the substance to be detected on the sampling swab SZ is eluted under the action of the rotating shear force, and under the action of the heating and extrusion unit, through the mechanical One or a combination of methods such as fragmentation,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a rapid detection method for a sampling swab and a fully-integrated analysis system for a swab sampled sample. The system comprises a pretreatment unit configured to store, elute and split the sampling swab to separate nucleic acid; a mixing unit configured to uniformly mix the nucleic acid separated by splitting with a reaction reagent; a sample splitting and amplification unit configured to distribute a mixture of a sample and the reaction reagent to each detection unit to realize multi-channel parallel nucleic acid amplification; an optical detection unit configured to detect a nucleic acid amplification result of the sample splitting and amplification unit; a temperature control unit configured to perform heating temperature control on the pretreatment unit and the sample splitting and amplification unit; a motion control unit configured to control the motion of the pretreatment unit, the mixing unit and the sample splitting and amplification unit; and a signal storage processing and analyzing unit, a wired or wireless communication interface, a display terminal and a cloud big data server. After sampling, the swab is loaded into a storage tube at one time without uncovering anymore, and the whole process is closed, so that the problems of infection of subsequent operators, cross contamination in the whole process and the like are effectively avoided.

Description

technical field [0001] The invention relates to the technical field of swab sampling biomedicine, in particular to a rapid detection method for swab sampling and a fully integrated analysis system for swab sampling. Background technique [0002] Swab sampling is widely used in biomedical scientific research, clinical medicine, health and epidemic prevention. The use process of conventional swab sampling is as follows: First, by manual means, use swabs to repeatedly test the parts of the human body where samples need to be extracted (such as throat, mouth, nasal cavity, ear canal, eyes, skin, vagina, anus or intestinal tract, etc.). Scratch and rub to transfer the sample attached to the human body to the swab; then, immerse the swab head in the special reagent in the swab storage tube, and manually wash it back and forth or use external forces such as vibration and ultrasound to remove the swab head. The sample attached to the swab head is eluted and put into the special rea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61B10/00C12Q1/6844C12M1/34C12M1/38
CPCA61B10/00C12Q1/6844
Inventor 黄国亮符荣鑫吕文琦杜文莉单晓晖靳翔宇林雪
Owner TSINGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com