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Method for simultaneously detecting isbiflorin and biflorin in medicinal material of Euscaphis konishii Hayata

A technology of Cavus sliver and medicinal materials, which is applied in the field of quantitative detection of Chinese medicinal materials, can solve the problems of complex chromatographic conditions, unclear anti-inflammatory activity, cumbersome operation, etc., and achieve simple operation, short time consumption, and good separation Effect

Pending Publication Date: 2017-07-28
FUJIAN AGRI & FORESTRY UNIV +1
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The method is cumbersome to operate, and the chromatographic conditions are complicated; the anti-inflammatory activity of the five components determined is not clear, and the total content is the highest in Soxhlet extraction, which is 0.5937mg / g, and the content in the medicinal material is less than 6 / 10,000

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] Example 1 Grind the dried medicinal material of Corvus dentata pericarp into a coarse powder, pass through a No. 3 sieve; weigh 1.0 g of the pericarp coarse powder, accurately weigh it, put it in a stoppered Erlenmeyer flask, accurately add 30 ml of 70% ethanol, and seal it tightly. , weighed, ultrasonically treated (power 140 W, frequency 42kHz) for 30 min; medicinal materials were treated with ultrasonic, allowed to cool, weighed again, made up for the lost weight with methanol, shaken, filtered, and the filtrate was taken as have to. Take 5 μl of the filtrate and inject it into Waters W2690 / 5-W2998 high performance liquid chromatograph; chromatographic conditions: Dikma DiamonsilC18 column, 250×4.6nm, 5μm, column temperature 30°C, flow rate 1.0 ml, mobile phase volume ratio methanol:water is 20 : 80, detection wavelength 234 nm. Through liquid phase analysis and linear regression equation calculation, the measured contents of isbiflorin and biflorin in the pericarp ...

example 2

[0024] Example 2 Weigh 1.0 g of the dried root powder of S. sylvanis, and operate according to the method of Example 1. The contents of isbiflorin and biflorin in S. sylvestris root are measured to be 0.35 mg / g and 0.41 mg / g, respectively.

example 3

[0025] Example 3 Weigh 1.0 g of the dried leaf powder of S. sylvanis, operate in the manner of Example 1, and measure the contents of isbiflorin and biflorin in the root of S. sylvestris to be 0.27 mg / g and 0.36 mg / g, respectively.

[0026] Weigh 1.0 g of the coarse powder of the dried seeds of S. sylvanis, and operate in the manner of Example 1, and the contents of isbiflorin and biflorin in the root of S. sylvestris are measured to be 0.44 mg / g and 0.13 mg / g, respectively.

[0027] Weigh 1.0 g of the coarse powder of the dried branches of Arvenia dentata, operate in the manner of Example 1, and measure the contents of isbiflorin and biflorin in the root of Arvenia rotundum to be 0.27mg / g and 0.33mg / g respectively.

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PUM

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Abstract

The invention discloses a method for simultaneously detecting isbiflorin and biflorin in a medicinal material of Euscaphis konishii Hayata. The method only comprises crushing dry Euscaphis konishii Hayata, carrying out ultrasonic extraction, filtering the extract and carrying out high performance liquid chromatography. The method can simultaneously determine the two components in the plant, is easy to operate and has the advantages of good separation degree, high accuracy and short time.

Description

technical field [0001] The invention provides a method for simultaneously detecting the content of isbiflorin and biflorin in the medicinal material of Corvus dentata, belonging to the technical field of quantitative detection of Chinese medicinal materials. Background technique [0002] The medicinal material of Crow's Toon is derived from the plant Crow's Crow in the province of oil family Euscaphis japonica and Crowwood Euscaphis konishii , its roots, branches, peels, and seeds can be used as medicine. Our research group has been committed to the development and utilization of the medicinal plant resources of Arvenia dentata. In the early stage, it has broken through the bottleneck in the production of C. scallopedus seedlings, and with the support of the National Agricultural Science and Technology Achievements Transformation Fund Project, it has successively established more than 2,000 mu of artificial forests in Nanping, Sanming, Fuzhou and other places in Fujian. ...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 倪林梁文贤邹双全黄维邹小兴
Owner FUJIAN AGRI & FORESTRY UNIV
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