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Method for manufacturing diapause transgenic silkworms through immediate corona

A transgenic and diapause technology, applied in animal husbandry and other directions, can solve problems such as environmental pollution and uneven discharge, and achieve the effects of simple and convenient operation, low cost and high efficiency

Inactive Publication Date: 2017-08-11
广西壮族自治区蚕业技术推广总站 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, researchers at that time invented the method of corona to relieve silkworm diapause, in order to solve the environmental pollution and cost problems caused by pickling in the production process of silkworm cocoons, and encountered problems such as uneven discharge in production and application that could not be solved. It has not been promoted and utilized, and no one will conduct subsequent research

Method used

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  • Method for manufacturing diapause transgenic silkworms through immediate corona
  • Method for manufacturing diapause transgenic silkworms through immediate corona

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Using the silkworm diapause variety "932" as the raw material, the silkworm eggs obtained by normal rearing were accelerated according to the conventional method used in production: the temperature was 27°C, and the relative humidity was 85%. In order to make the hatching tidy, the silkworm eggs are wrapped in black cloth after being lighted green, and the silkworm eggs are taken out two days later to collect ants. Raise the ant silkworms harvested by conventional methods: small silkworm stage (1-3 ages) 25-28°C, relative humidity 75-85%; large silkworm stage (4-5 ages) 23-26°C, relative humidity 70-80% , ventilate and ventilate; use natural light for all ages, and feed mulberry leaves three times a day. Or the whole age can be raised at room temperature. Mature silkworms are transferred to an environment around 25°C to form clusters and form cocoons. After pupation, the cocoons are plucked, and the male and female moths are identified by pinching them with a knife, an...

Embodiment 2

[0043] Using the silkworm diapause Japanese variety "7532" as the raw material, the silkworm eggs obtained by normal rearing were accelerated according to the conventional method used in production: temperature 27°C, relative humidity 85%. In order to make the hatching tidy, the silkworm eggs are wrapped in black cloth after being lighted green, and the silkworm eggs are taken out two days later to collect ants. Raise the ant silkworms harvested by conventional methods: small silkworm stage (1-3 ages) 25-28°C, relative humidity 75-85%; large silkworm stage (4-5 ages) 23-26°C, relative humidity 70-80% , ventilate and ventilate; use natural light for all ages, and feed mulberry leaves three times a day. Or the whole age can be raised at room temperature. Mature silkworms are transferred to an environment around 25°C to form clusters and form cocoons. After pupation, the cocoons are plucked, and the male and female moths are identified by pinching them with a knife, and placed s...

Embodiment 3

[0045] Using the diapause Japanese variety "Dazao" of silkworm as the raw material, the silkworm eggs obtained by normal rearing were accelerated according to the conventional method used in production: temperature 27°C, relative humidity 85%. In order to make the hatching tidy, the silkworm eggs are wrapped in black cloth after being lighted green, and the silkworm eggs are taken out two days later to collect ants. Raise the ant silkworms harvested by conventional methods: small silkworm stage (1-3 ages) 25-28°C, relative humidity 75-85%; large silkworm stage (4-5 ages) 23-26°C, relative humidity 70-80% , ventilate and ventilate; use natural light for all ages, and feed mulberry leaves three times a day. Or the whole age can be raised at room temperature. Mature silkworms are transferred to an environment around 25°C to form clusters and form cocoons. After pupation, the cocoons are plucked, and the male and female moths are identified by pinching them with a knife, and plac...

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Abstract

The invention discloses a method for manufacturing diapause transgenic silkworms through immediate corona. A high voltage electric field is utilized for performing immediate corona stimulation on diapause silkworm eggs obtained after transgenic injection so as to remove diapause, and an incubation period is directly entered. After the method is applied for treatment, positive individuals are screened through fluorescence detection to be reared for subculture, and the problems that transgenic operation of silkworm diapause varieties is complex and efficiency is low can be effectively solved. A research shows that the corona treatment egg incubation rate reaches up to 99%, the Chinese variety is as good as the Japanese variety in effect, and no influence is caused on growth, development, cocooning and other economic characters of later period silkworms. In general, the method is easy and convenient to implement, low in cost, high in efficiency, safe, free of pollution and applicable to various silkworm varieties, and provides powerful technical support for manufacturing of transgenic silkworms.

Description

technical field [0001] The invention belongs to the technical field of transgenic silkworms, in particular to a method for producing diapause transgenic silkworms by instant corona. Background technique [0002] The silkworm is an important economic insect and a model organism of Lepidoptera. An important physiological phenomenon in silkworms is transgenicity, that is, the number of generations that occurs in a year. Monomorphic silkworm species only go through one generation a year, and then the eggs laid enter a long diapause period until the second year when diapause is released under suitable temperature and humidity conditions and start to develop; There are two generations in the natural environment, and the method of soaking in hydrochloric acid can artificially release diapause and control the number of reproductive generations; polymorphic silkworm species have more than three generations within a year, and non-diapause polymorphic species have no diapause period. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/04
Inventor 张雨丽张桂征赵爱春龙定沛闭立辉向仲怀韦伟韦博尤黄文功鲁成苏红梅黄玲莉
Owner 广西壮族自治区蚕业技术推广总站
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