Novel pediococcus acidilactici and application thereof

A technology of Pediococcus lactis and porcine-sourced lactic acid tablets, which is applied to the new Pediococcus lactis and its application fields, can solve the problems that do not involve the adhesion of Pediococcus lactis to epithelial cells, and do not involve the verification effect, etc., and achieve a strong inhibitory effect , good antibacterial, strong adhesion effect

Active Publication Date: 2017-08-11
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above patents do not involve the adhesion of Pediococcus lactis to epithelial cells, nor do they involve animal experiments and other aspects of verification effects

Method used

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  • Novel pediococcus acidilactici and application thereof
  • Novel pediococcus acidilactici and application thereof
  • Novel pediococcus acidilactici and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Isolation, screening and identification of Pediococcus lactis

[0038] From the fresh feces of weaned piglets from healthy Beijing black pigs, aseptically weighed an appropriate amount and added it to sterile normal saline, oscillated evenly and made a ten-fold gradient dilution, spread it on the MRS medium plate, anaerobic tank method 37 Cultivate at ℃ for 24-48 hours. Pick milky white colonies from them for preliminary screening, all Gram-stained positive, H 2 o 2 The strains with negative enzyme test and negative contact enzyme can be preliminarily determined to be lactic acid bacteria. The strains obtained from the preliminary screening are tested for acid resistance, bile salt resistance, antibacterial and other tests, and finally a strain of lactic acid bacteria ZPA017 with excellent performance is screened out and sent to China General Microbiology Bacteria Co., Ltd. Species Collection Center (CGMCC) for identification, cell morphology characteristics...

Embodiment 2

[0045] Example 2 Study on the characteristics of Pediococcus lactis

[0046] 1. Determination of acid resistance

[0047] The activated Pediococcus acidilactici (Pediococcus acidilactici) ZPA017 obtained in Example 1 was inserted into the liquid MRS medium with a pH of 2, 3 and 4 respectively at an inoculum size of 1%, and incubated at 37°C for 2, 4 and 6 hours respectively , with 0 hour as the control, the number of viable bacteria was measured, and the survival rate was calculated. The test results are shown in Table 2.

[0048] Table 2 Acid resistance of Pediococcus lactis (%)

[0049]

[0050] As can be seen from the results, this Pediococcus lactis acts in the acidic environment of pH=2 for 2 hours, and its survival rate is 98.45%, and the survival rate at the time of 4 hours is 82.17%, and the survival rate at the time of 6 hours is 77.91%; In the environment of 3, the survival rate at 2 hours was 100.78%, the survival rate at 4 hours was 88.33%, and the survival r...

Embodiment 3

[0072] Embodiment 3, the preparation of Pediococcus lactis preparation

[0073] 1. Fermentation of Pediococcus lactis

[0074] The Pediococcus acidilactici ZPA017 (preservation number: CGMCC No.9040) obtained in Example 1 was inoculated in the MRS liquid medium with an inoculum size of 1%, and cultured at 37° C. for 24 hours, so that seeds were obtained after 2 generations of activation Culture: Inoculate the seed culture with 5% inoculum in a 50L fermenter equipped with 30L optimized liquid medium, set the temperature at 37°C, rotate at 100rpm, and maintain a constant pH of 4.8, when fermented to 16 hours Feed feeding was started, and 200 mL of feeding liquid was added to the fermenter at a time, and then feeding was carried out every 2 hours for a total of 5 feedings; when the pH of the fermentation broth no longer decreased, the fermentation was terminated.

[0075] Wherein, the optimized liquid medium is as described in Example 2. The feeding liquid is glucose solution w...

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Abstract

The invention discloses a novel strain ZPA017 of pediococcus acidilactici. The novel strain ZPA017 is assigned the accession number CGMCC No. 9040, and the novel strain ZPA017 has the advantages of being resistant to acid and bile salt, high in adhesion and good in antibacterial property. The invention further discloses a preparation containing the pediococcus acidilactici ZPA017. The preparation serves as a feed additive to feed weaned piglets and can inhibit reproduction of harmful bacteria and regulate intestinal microecology balance, and the preparation has effects of enhancing organism immunity and improving production performance.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a novel Pediococcus lactis and application thereof. Background technique [0002] Because the digestive system and physiological functions of weaned piglets are not yet fully developed, they are easily affected by stress factors such as weaning, feed nutrition, and feeding management, and the intestinal flora disorder occurs, causing diarrhea in piglets, which in turn leads to decreased feed intake and growth retardation. Even death, bring great loss to pig production. [0003] Pediococcus acidilactici (Pediococcus acidilactici) is a lactic acid bacterium in the Pediococcus genus, which is commonly used in the fermentation of meat, dairy products, vegetables and silage, etc. Its main advantages are: rapid growth, the ability to produce a large amount of organic acids such as lactic pH value; produce organic acids and antibacterial substances, which can inhibit the growth ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A23K10/18A23K50/30C12R1/01
CPCC12N1/04C12N1/20C12N1/205C12R2001/01
Inventor 刘辉季海峰张董燕王四新王晶张伟王雅民
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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