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A special high-selenium feed for Daphnia and its application method

A technology of daphnia and feed, applied in animal feed, application, additional food elements, etc., can solve the problems of selenium yeast spirulina feed, low cultivation cost, short growth cycle, etc., and achieve mature large-scale breeding technology, The effect of low culture cost and short growth cycle

Active Publication Date: 2020-03-20
ANHUI UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Li Hechao and others used chlorella yeast powder to achieve large-scale cultivation of Daphnia. Spirulina has similar nutritional value, and the cultivation cost is low, the growth cycle is short, and the large-scale cultivation technology is mature. Selenium yeast has not yet been seen in the market. Report on Spirulina Feed

Method used

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  • A special high-selenium feed for Daphnia and its application method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: the selenium yeast spirulina feed culture of Daphnia sinensis:

[0047] Put the spirulina species into the spirulina culture medium for cultivation, the inoculation amount is 10% (V / V), the temperature is 25 degrees, and the light intensity is 4000Lx. ), prepare 5L of culture medium, add appropriate amount of daphnia (selenium concentration 0.04ug / ml), after 48 hours, compared with daphnia cultured in pond water, its survival rate increases by 12.18%, egg laying rate increases by 20.03%, GSH-PX activity Increased by 170.26%, T-SOD activity increased by 85.67%.

Embodiment 2

[0048] Embodiment 2: the selenium yeast spirulina feed culture of daphnia

[0049] Put the spirulina species into the spirulina culture medium for cultivation, the inoculation amount is 10% (V / V), the temperature is 25 degrees, and the light intensity is 4000Lx. g), prepare 5L of culture medium, add appropriate amount of daphnia (selenium concentration 0.04ug / ml), after 48 hours, compared with daphnia cultured in pond water, its survival rate increased by 10.53%, egg laying rate was 18.72%, GSH-PX activity Increased by 175.58%, T-SOD activity increased by 84.15%.

Embodiment 3

[0050] Embodiment 3: the selenium yeast spirulina feed culture of laboratory daphnia

[0051] Put the spirulina species into the spirulina culture medium for cultivation, the inoculum size is 10% (V / V), the temperature is 25 degrees, and the light intensity is 4000Lx. After 8 days of cultivation, take 1g of the cultured spirulina and feed yeast 2.6mg (the selenium content is 1.5mg / g), prepare 100ml of culture medium, add appropriate amount of daphnia (selenium concentration 0.04ug / ml), after 48 hours, compared with daphnia cultured in green water, its survival rate increased by 14.36%, and its egg laying rate was 22.90%, GSH-PX The activity increased by 178.64%, and the T-SOD activity increased by 87.29%.

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Abstract

The invention discloses a high-selenium feed specially used for water flea, and applications thereof. Compared with water flea cultured with pond water, the survival rate of water flea cultured with the high-selenium feed can be increased by 10 to 15%, egg yield is increased by 18 to 23%, the activity of GSH-PX in water flea is increased by 163 to 180%, T-SOD activity is increased by 83.67 to 85.72%. The high-selenium feed specially used for water flea is high in selenium content, low in raw material culturing cost, is capable of shortening growth period, can be used for culturing water flea with high fertility, stress resistance, and nutritional value, and is suitable for commericial production of water flea; and large-scale culturing technology with the high-selenium feed is mature.

Description

technical field [0001] The invention belongs to the field of feed development and application, and relates to a special high-selenium feed for daphnia and a use method thereof. Background technique [0002] Daphnia, commonly known as red worms, is a good feed for poultry, livestock, and fish. It does not pollute water and prevent parasitic diseases. Its dry product contains 60.4% protein, 21.8% fat, 1.1% sugar, 4.27% tyrosine, 3.62% tryptophan, 10.92% arginine, 2.69% histidine, 1.17% cystine and 3.45% methionine. It is also rich in vitamins and minerals, and its nutritional value is superior to fishmeal. Daphnia is irreplaceable as fish feed in many aspects. For example, sturgeon and salmon with high economic value, leather beard catfish, and juvenile fish must eat daphnia, otherwise the survival rate is extremely low; precious goldfish must also be fed with fleas, otherwise they will easily die. Suffering from indigestion. [0003] Daphnia is also one of the model organi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/80A23K20/20
Inventor 黄蓓陈凌云汤成华张意张玉英
Owner ANHUI UNIVERSITY
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