Application of protoheme and beta-glucuronidase in bronchial epithelial cell heterogeneity hyperplasia detection and kit

A technology of bronchial epithelium and detection kit, which is applied in the direction of analyzing materials through chemical reactions and observing the influence of chemical indicators on materials, etc., which can solve the problem of low sensitivity and specificity of detection results and screening The method is cumbersome, and the diagnosis result of lung cancer is not accurate, etc., so as to improve the accuracy

Inactive Publication Date: 2017-08-15
山东瓦博格生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the current screening methods for detecting heterogeneous proliferation of bronchial epithelial cells are relatively cumbersome, and the corresponding detection is based on a single marker. The sensitivity and specificity of the detection results are not high, and the diagnosis of lung cancer is not accurate.

Method used

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  • Application of protoheme and beta-glucuronidase in bronchial epithelial cell heterogeneity hyperplasia detection and kit
  • Application of protoheme and beta-glucuronidase in bronchial epithelial cell heterogeneity hyperplasia detection and kit
  • Application of protoheme and beta-glucuronidase in bronchial epithelial cell heterogeneity hyperplasia detection and kit

Examples

Experimental program
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Embodiment 1

[0061] This embodiment provides a bronchial epithelial cell heterogeneous hyperplasia detection kit, which uses proheme and β-glucuronidase as markers for joint detection. The detection results of proheme and β-glucuronidase are used to judge the heterogeneous proliferation of bronchial epithelial cells in the sample.

[0062] The bronchial epithelial cell heterogeneous hyperplasia detection kit provided in this embodiment includes a proheme detection reagent membrane for detecting proheme and a β-glucuronidase for detecting β-glucuronidase Detection reagent membrane.

[0063] Wherein, the original hemoglobin detection reagent film provided by the present invention is made according to the following method:

[0064] 1.1. Make phosphate buffer. The buffer is 1.75mol / L, pH 4.5 phosphate buffer.

[0065] 1.2. Add quantitative polyvinylpyrrolidone K30 to the above solution to obtain the first mixed solution, and stir until completely dissolved; wherein, the dosage of polyvinylp...

Embodiment 2

[0095] This embodiment provides a bronchial epithelial cell heterogeneous hyperplasia detection kit, which uses proheme and β-glucuronidase as markers for combined detection. Through the detection results of proheme and β-glucuronidase, it is used to judge the heterogeneous proliferation of cells in the sample.

[0096] The bronchial epithelial cell heterogeneous hyperplasia detection kit provided in this embodiment includes a proheme detection reagent membrane for detecting proheme, and a β-glucuronidase for detecting β-glucuronidase Detection reagent membrane and sample pretreatment solution for sample processing.

[0097] Wherein, the preparation method of the original heme detection reagent membrane, the β-glucuronidase detection reagent membrane and the sample pretreatment solution can refer to Example 1.

[0098] The effect of the detection kit for bronchial epithelial cell heterogeneity provided in this example is the same as that in Example 1.

Embodiment 3

[0100] This example provides verification of the sensitivity, specificity and Youden index of proheme and β-glucuronidase as combined markers for detecting bronchial epithelial cell heterogeneity.

[0101] Sensitivity: also known as sensitivity, refers to the proportion of people who are actually sick can be correctly identified as patients by the screening method; specificity: refers to the proportion of people who are actually disease-free by the screening method. The proportion judged as non-patients.

[0102] 3.1 Sensitivity Verification

[0103] 3.1.1 Source of specimens: 100 sputum specimens of lung cancer patients collected in the clinical laboratory of a medical institution. Lung cancer was diagnosed by chest CT or MRI.

[0104] 3.1.2 Detection method: The bronchial epithelial cell heterogeneity detection kit provided in Example 2 was used to conduct combined detection of proheme and β-glucuronidase on the above 100 samples, and provided according to Example 1 Inter...

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Abstract

The invention discloses an application of protoheme and beta-glucuronidase in bronchial epithelial cell heterogeneity hyperplasia detection and a kit and belongs to the technical field of liquid biopsy pathology detection. The protoheme and the beta-glucuronidase are applied to the field of bronchial epithelial cell heterogeneity hyperplasia detection as associated markers for the first time, and people are surprised to find that the sensitivity, the specificity and the Youden index of detection employing the protoheme and the beta-glucuronidase as the associated markers are obviously improved in comparison with those of detection employing the protoheme or the beta-glucuronidase as the marker separately, so that the protoheme and the beta-glucuronidase are conducive to improvement of the accuracy of the diagnosis result on a lung cancer caused by bronchial epithelial cell heterogeneity hyperplasia as the associated markers.

Description

technical field [0001] The invention relates to the technical field of liquid biopsy pathological detection, in particular to the application and kit of the combination of proheme and β-glucuronidase in the detection of bronchial epithelial cell heterogeneity. Background technique [0002] The result of heterogeneous proliferation of bronchial epithelial cells is lung cancer. According to statistics, the incidence of lung cancer in my country increases by 26.9% every year. Lung cancer has become the first cause of death from malignant tumors in my country, accounting for 22.7% of all malignant tumor deaths. Due to air pollution and other factors, the incidence and mortality continue to rise rapidly. From 2000 to 2005, the incidence of lung cancer in China is estimated to have increased by 120,000, of which male lung cancer patients increased from 260,000 in 2000 to 330,000 in 2005, and female lung cancer patients increased from 120,000 to 170,000 in 2005. It is estimated tha...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 温鹏
Owner 山东瓦博格生物技术有限公司
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