Method for analyzing metabolism regulation mechanism of carotenoid in maize grains based on dynamic correlation between co-expression patterns of gene pairs

Abstract

The invention belongs to the field of functional genomics and particularly relates to a method for analyzing a metabolism regulation mechanism of carotenoid in maize grains based on dynamic correlation between co-expression patterns of gene pairs. The method comprises the following steps: downloading 13 key genes in a carotenoid metabolism pathway from a maize genome database maizeGDB; collecting grain transcripts obtained after pollinating maize inbred lines for 15 days, and sequencing to obtain gene expression level data, and extracting expression level data of the 13 key genes; building a dynamic correlation analysis LA model; and analyzing and identifying a regulation relation between the key genes in the pathway utilizing a dynamic correlation analysis. The method is capable of excavating the regulation relation between the 13 key genes in the carotenoid metabolism pathway based on dynamic correlation analysis, analyzing the regulation mechanism of synthesis and accumulation of carotenoid in grains and providing optimal gene combination for molecular-marker assistant breeding, and has important significance for increasing carotenoid in maize and improving maize quality.

Description

technical field [0001] The invention belongs to the field of functional genomics, and specifically relates to a method for analyzing the regulation mechanism of carotenoid metabolism in maize grains based on the dynamic correlation of gene pair co-expression patterns. Background technique [0002] Corn is a crop rich in carotenoids. Among the main food crops, only corn contains vitamin A sources and lutein carotenoids that are beneficial to the human body. Animals cannot synthesize carotenoids themselves and must obtain them from food. Obtain. Corn kernels mainly include five carotenoids: lutein, zeaxanthin, β-cryptoxanthin, α-carotene and β-carotene, about 95% are stored in the endosperm, and 2-4% are stored in the corn In bran, the content of total carotenoids in germ only accounts for 1%. The accumulation of carotenoids in corn begins 10-15 days after pollination. Yellow-grained corn is rich in carotenoids and has a wide range of heritability. It is a good material for ...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the gaps in the research on the molecular mechanism of carotenoid accumulation existing in the existing problems, the present invention provides a method for analyzing the regulation mechanism of carotenoid metabolism in corn grain based on the dynamic correlation of gene pair co-expression patterns. Association analysis, using the known key genes in the carotenoid metabolism pathway of maize grains as the target gene, to identify the mutual regulatory relationship between key genes, so as to analyze the regulation mechanism of carotenoid metabolism in maize grains, and provide a basis for the biochemical enhancement of maize grain carotenoids. High-quality gene combinations, and provide ideas and methods for the study of the regulatory relationship between key genes of other agronomic traits in maize

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