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Obese population-specific biomarker composition and use thereof

A biomarker and biomarker technology, applied in the field of biomarker compositions, can solve the problems of poor sensitivity and specificity of biomarkers

Active Publication Date: 2019-01-18
BGI SHENZHEN CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the shortcomings of poor sensitivity and specificity of biomarkers in existing obesity diagnostic methods, the problem to be solved by the present invention is to provide a biomarker combination (i.e. biomarker composition) that can be used for obesity diagnosis and disease risk assessment. ), and methods for risk assessment and diagnosis of obesity

Method used

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  • Obese population-specific biomarker composition and use thereof
  • Obese population-specific biomarker composition and use thereof
  • Obese population-specific biomarker composition and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] 1.1 Sample collection: The morning blood of the volunteers was collected and immediately stored in a -80°C low-temperature refrigerator. A total of 84 plasma samples were collected in the obese group and 104 plasma samples in the control group.

[0083] 1.2 Processing of samples: thaw frozen samples at room temperature, take 500 μL of plasma samples and transfer them to 2.0 mL centrifuge tubes, add 1000 μL of methanol to dilute, centrifuge at 10000 rpm for 5 min, and set aside.

[0084] 1.3 Liquid chromatography-mass spectrometry analysis

[0085] equipment

[0086] HPLC-MS-LTQ Orbitrap Discovery (Thermo, Germany)

[0087] Chromatographic conditions

[0088] Chromatographic column: C18 column (150mm×2.1mm, 5μm); mobile phase A: 0.1% formic acid aqueous solution, mobile phase B: 0.1% formic acid acetonitrile solution; gradient elution program: 0~3min, 5% B, 3~36min, 5%~80%B, 36~40min, 80%~100%B, 40~45min, 100%B, 45~50min, 100%~5%B, 50~60min, 5%B; flow rate: 0.2mL / m...

Embodiment 2

[0096] 2.1 Sample collection: The morning blood of the volunteers was collected and immediately stored in a -80°C low-temperature refrigerator. A total of 84 plasma samples were collected in the obese group and 104 plasma samples in the control group.

[0097] 2.2 Processing of samples: thaw frozen samples at room temperature, take 500 μL of plasma samples and transfer them to 2.0 mL centrifuge tubes, add 1000 μL of methanol to dilute, centrifuge at 10000 rpm for 5 min, and set aside.

[0098] 2.3 Liquid chromatography-mass spectrometry analysis

[0099] equipment

[0100] HPLC-MS-LTQ Orbitrap Discovery (Thermo, Germany)

[0101] Chromatographic conditions

[0102] Chromatographic column: C18 column (150mm×2.1mm, 5μm); mobile phase A: 0.1% formic acid aqueous solution, mobile phase B: 0.1% formic acid acetonitrile solution; gradient elution program: 0~3min, 5% B, 3~36min, 5%~80%B, 36~40min, 80%~100%B, 40~45min, 100%B, 45~50min, 100%~5%B, 50~60min, 5%B; flow rate: 0.2mL / m...

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Abstract

Disclosed is a blood plasma specific metabolite profile, in particular a biomarker composition obtained via obese population blood plasma specific metabolite profile screening. Also disclosed are a use of the biomarker composition for obesity risk assessment, diagnosis, early diagnosis and pathological staging, and an obesity risk assessment, diagnosis, early diagnosis and pathological staging method. The biomarker composition of the present invention may be used for the early diagnosis of obesity.

Description

technical field [0001] The present invention relates to a plasma-specific metabolite profile, in particular to a biomarker composition screened from the plasma-specific metabolite profile of an obese subject. The present invention also relates to the use of the biomarker composition in risk assessment, diagnosis, early diagnosis and pathological staging of obesity, and methods for risk assessment, diagnosis, early diagnosis and pathological staging of obesity. Background technique [0002] Obesity, also known as obesity, is a chronic metabolic disease caused by multiple factors and closely related to genetics, environment, and lifestyle [1]. With the development of society and the improvement of living standards, the incidence of obesity has risen sharply and become a global problem. According to statistics, the prevalence of obesity in Europe and the United States is about 20%, and about 65% of Americans are either overweight or obese [2], and the prevalence of obesity amo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/70
CPCG01N33/68G01N33/70
Inventor 冯强刘志鹏陈晓敏范艳群郭珍玉李光磊王俊
Owner BGI SHENZHEN CO LTD
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