47 results about "Metabolite profiling" patented technology

An apparatus and method analyzes a metabolic profile in a biological sample. The apparatus includes an input unit for inputting the drugs and/or metabolites to be screened; a controlling unit for determining a parameter set for metabolites preparation and for mass spectrometry analysis depending on the input of the kind of metabolites to be screened; a treatment unit for preparing the metabolites to be screened depending on the determined parameter set; a mass spectrometer for performing mass spectrometry analysis on prepared metabolites depending on the parameter set; a database for storing results of analyzing and parameter sets for metabolite preparation and for mass spectrometry analyses; and an evaluation unit for evaluating the results of mass spectrometry by use of reference results stored in the database to output an analysis of the metabolites profile.

The invention discloses a serum specificity metabolite spectrum for a patient with a lung cancer and a building method thereof, and particularly discloses a method for carrying out liquid-phase/gas-phase chromatography mass spectrometry metabonomics analysis based on the patient suffering from the lung cancer. The method comprises the following steps: collecting fasting blood samples of a healthy and normal detected person and the patient suffering from the lung cancer 7 days before and after an operation, separating by a chromatogram column after processing; extracting and aligning original data of an instrument after adopting mass spectrometry analysis; building a mathematical model to analyze the metabolites spectrum by adopting multivariable data statistical software; comparing with the difference between an average person with the metabolites spectrum of the patient with the lung cancer, so as to determine the specificity metabolite spectrum. By adopting the method disclosed by the invention, the mutation status of the metabolite of the patient with the lung cancer can be generally and comprehensively reflected, and the serum specificity metabolite spectrum can be applied to early diagnosis of the lung cancer or a beneficial technical support is supplied for prognosis.

Embodiments include methods for generating a metabolite profile of a stool sample and methods of assessing the status of a subject using the metabolic profile derived from a stool sample.

A monitoring test for recurrent breast cancer with a high degree of sensitivity and specificity is provided that detects the presence of a panel of multiplicity of biomarkers that were identified using metabolite profiling methods. The test is capable of detecting breast cancer recurrence about a years earlier than current available monitoring diagnostic tests. The panel of biomarkers is identified using a combination of nuclear magnetic resonance (NMR) and two dimensional gas chromatography-mass spectrometry (GC×GC-MS) to produce the metabolite profiles of serum samples. The NMR and GC×GC-MS data are analyzed by multivariate statistical methods to compare identified metabolite signals between samples from patients with recurrence of breast cancer and those from patients having no evidence of disease.

The invention provides a method for optimizing a fermentation medium by improving rapamycin production by using metabolic profiling analysis, which researches dynamic changes and differences of bacteria intracellular metabolites under the culture conditions of initial fermentation medium and optimized fermentation medium by using the methods of dynamic detection and metabolic profiling analysis. The analysis result of partial least squares indicates that the bacteria primary metabolite profile and the rapamycin production have correlation and a further dynamic adding measure is provided to the medium by analyzing the dynamic changes of the key compounds combining related processes. Due to the research of S.hygroscopicus U1-6E7 fermentation medium, the rapamycin production is improved from 196mg/L to 307mg/L and is improved by 56.6%, which indicates that rational guidance of optimization of fermentation mediums by dynamic detection combining the metabolite profile analysis method is capable of successfully improving fermentation level of antibiotic and providing a new idea for optimization of fermentation mediums.

The invention discloses a metabonomics testing method for spermine-regulated mammalian intestinal development. A novel way is provided for accurately evaluating an overall nutrition metabolism effect for promoting the animal intestinal development through spermine based on the metabonomics technology. Change of an animal intestinal metabolite spectrogram can fully reflect comprehensive influence in aspects such as nutrition, individual and environment and timely and sensitively reflect correlation among animal spermine nutrition and metabolism conditions thereof as well as intestinal development. According to the method, biological markers related to the intestinal spermine nutrition process can be screened, the intrinsic quality of the spermine nutrition process is disclosed, the method has important theory and practice significance for perfecting digestive absorption functions of the animals, optimizing the feed formula and improving healthy growth of the animals, and the gaps that the overall nutrition metabolism conditions of animal intestinal development cannot be evaluated by combining with the metabonomics and the animal intestinal development cannot be improved are filled. Moreover, the research result proves that the method has actual application values in breeding industry.

The present invention relates to a method of identifying a gene encoding a polypeptide or polypeptide isoform which is substantially more active in either a proanthocyanidin (PA) or anthocyanin (ANT) pathway of a plant, said method including providing material from said plant; and an oligonucleotide probe capable of hybridizing with RNA from a gene encoding a polypeptide which is active in a flavonoid biosynthetic pathway; extracting RNA from said plant material; hybridizing the oligonucleotide probe with the RNA to generate an expression profile; measuring PA and/or ANT levels in said plant material to generate a metabolic profile; comparing said expression profile with said metabolite profile to identify said gene encoding a polypeptide or polypeptide isoform which is substantially active in either a PA or ANT pathway.

The present invention relates to methods of determining if a subject has an increased risk of suffering from memory impairment. The methods comprise analyzing at least one plasma sample from the subject to determine a value of the subject's metabolite profile and comparing the value of the subject's metabolite profile with the value of a normal metabolite profile. A change in the value of the subject's metabolite profile, over normal values is indicative that the subject has an increased risk of suffering from memory impairment compared to a normal individual.

The invention provides a method for testing a spectrum of o-dihydroxy metabolites in urine. Solid-phase extraction is carried out by using phenylo boric acid so as to purify and enrich the o-dihydroxy metabolites; and liquid chromatography-mass spectrometry is adopted to test a characteristic spectrum of the o-dihydroxy metabolites. Concentration by centrifuging is carried out by collecting formic acid-methanol water eluate; and residua are dissolved in water and analyzed after centrifugation. The invention provides a new method for testing the spectrum of the o-dihydroxy metabolites and lays a foundation for further discovering new diagnostic markers of breast cancer.

The invention relates to an amino acid metabolomics-based method for researching an anti-insomnia action mechanism of sugemule. Amino acid metabolome in each of tissues of the brains of para-chlorophenylalanine (PCPA) insomnic model rats before and after gavage with the sugemule is analyzed by applying a gas chromatography-mass spectrometer (GC-MS) method; endogenous compounds are identified by astandard fingerprint bank; changes of metabolite maps of all parts of the brains are analyzed and related metabolic markers are assayed by employing principal component analysis (PCA) and partial least squares-discrimination analysis (PLS-DA) modes; a targeting metabolomics research is conducted by applying liquid chromatograph-mass spectrometer (LC-MS) to explore content changes of neurotransmitters including 5-hydroxytryptamine, gamma-aminobutyric acid (GABA), glutamic acid (Glu) and the like in the brain tissues and related metabolites; by seeking for difference metabolites related with thecurative effect of the sugemule and metabolic pathways of the difference metabolites from brain tissue samples of clinical rats, the anti-insomnia action mechanism and an action site of the sugemuleare specified; and a metabolomics research means is applied for the first time by the amino acid metabolomics-based method to preliminarily confirm the action mechanism of the sugemule regulating andcontrolling a sleep-wake rhythm by a neurotransmitter system.

Metabolite profiles are generated from magnetic resonance images. These metabolite profiles indicate a topography of the metabolites in one or more anatomical regions. The magnetic resonance images can be metabolite images, such as those obtained using hyperpolarized ¹³C-MRI. The metabolite profiles can be generated by parcellating the magnetic resonance images into different regions, computing z-scores for those regions, and generating the metabolite profile based on the computed z-scores.

The invention provides methods and compositions for the modulation of proanthocynidin (“PA;” condensed tannin) production in plants. The methods of the invention allow creation of plants having novel phenotypes, such as alterations in levels of PA or in the types of tissues in which PAs are produced. Altered expression of PA in plants may be achieved, for instance without significantly affecting anthocyanin content or overall secondary metabolite profiles. Alternatively, expression of PA in plants may be achieved while also affecting anthocyanin content.

Devices, methods and systems are for identifying the cell type of an unknown microorganism. The device includes: an apparatus for culturing unknown organism(s), a diagnostic data acquisition tool and a computer program. The method includes: incubation of the sample with a growth medium (with or without toxins), and an analysis of the metabolites detected in the sample. The computer system compares the results collected from the device to reference metabolite profiles.

A magnetic resonance (MR) system for detecting concentrations of one or more metabolites in a volume of interest (VOI), the system including at least one controller which: may apply to the VOI a multiple quantum filter (MQF) Point Resolved Spetroscopy (PRESS) sequence comprising first and second 90° RF pulses, a third 90° RF pulse, first and second 180° adiabatic pulses, and a composite dual-band delay alternating with nutation for tailored excitation (DANTE) pulse train having a plurality of N block pulses (N being an integer), the DANTE pulse train situated in time between the first and second 90° RF pulses, the first and second 180° adiabatic pulses situated in time after third 90° RF pulse; detect MR Free Induced Decay (FID) signal emitted from the VOI; and/or reconstruct the detected MR FID signal to obtain metabolite spectrum information.

The invention provides methods and products to detect the presence of unidentified high grade prostate tumors in a subject with a Gleason score 7 prostate tumor. The method comprises obtaining a biological sample from a subject in need thereof, measuring a profile of metabolites in the biological sample, wherein the metabolites are differentially expressed in Gleason score 6 versus Gleason score 8 prostate tumors, and classifying the profile of the metabolites to assign a grade to the sample based on the profile of the metabolites.

A methods for altering the metabolite profile of a fermentation, by increasing flux through acetolactate. The methods comprises increasing production of one or more products derived from acetolactate. Further provided is a method for increasing the production of 2,3-butandiol by microbial fermentation of gaseous substrates, the method comprising providing a compound which inhibits one or more enzymes which convert acetolactate to branched chain amino acids to the fermentation. The present invention further provides methods for increasing the production of 2,3-butandiol relative to other fermentation products such as ethanol and acetic acid.