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Metabonomics testing method for spermine-regulated mammalian intestinal development

A technology of intestinal development and metabolomics, applied in the direction of analysis using nuclear magnetic resonance, can solve problems such as shortage, and achieve the effect of optimizing feed formula, improving digestion and absorption function, and improving healthy growth

Inactive Publication Date: 2014-07-09
SICHUAN AGRI UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the embodiments of the present invention is to provide a metabolomics test method for spermine to regulate intestinal development of mammals, aiming to solve the existing lack of combining metabolomics to evaluate the overall nutritional and metabolic status of animal intestinal development, and to improve animal digestion and absorption functions , raising the issue of its healthy growth

Method used

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  • Metabonomics testing method for spermine-regulated mammalian intestinal development
  • Metabonomics testing method for spermine-regulated mammalian intestinal development
  • Metabonomics testing method for spermine-regulated mammalian intestinal development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: the influence of spermine on intestinal development of young mammals;

[0045] 1. Materials and methods:

[0046] 1. Experimental animals and experimental design:

[0047] Forty SD female healthy suckling rats were divided into control group (normal saline) and treatment group (spermine), totally 4 groups. Spermine was administered to 8-day-old suckling rats for 3 days and 7 days, the dose of spermine was 0.2 μmol / gBW, and each treatment was repeated 10 times, with 1 suckling rat for each repetition;

[0048] 2. Feeding management:

[0049] All suckling rats and mother rats were housed in single cages in stainless steel cages. All utensils were washed and disinfected. During the experiment, the room temperature was controlled at 20°C-25°C, and the relative humidity was controlled at 40%-70%. During this period, the feeding, drinking, defecation, urination regularity and mental state of suckling rats and mother rats were observed;

[0050] 3. Sample col...

Embodiment 2

[0054] Example 2. Metabonomics research method of spermine regulating intestinal development of suckling rats

[0055] 1. Sample pretreatment and data collection:

[0056] 1. Sample preparation and testing:

[0057] Use an electronic balance to weigh about 60 mg of the intestinal tract, take the same part of the tissue, place them in 2ml EP tubes, add 1ml of 66.7% methanol solution and 30 tungsten carbide beads with a diameter of about 2mm, and break the tissue Carry out crushing on the instrument for 90s, then sonicate in an ice-bath ultrasonic cleaner for 3min, then centrifuge at 14500g for 10min at 4°C, absorb the supernatant and put it in a new 2ml EP tube, repeat the crushing and extraction process twice, The supernatants were collected using a vacuum centrifugal concentrator to evaporate the organic solvent and then freeze-dried using a freeze dryer. Add the freeze-dried tissue extract powder to 600 μL phosphate buffer (0.1M NaH 2 PO 4 / K 2 HPO 4 , 50% of D 2 O, p...

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Abstract

The invention discloses a metabonomics testing method for spermine-regulated mammalian intestinal development. A novel way is provided for accurately evaluating an overall nutrition metabolism effect for promoting the animal intestinal development through spermine based on the metabonomics technology. Change of an animal intestinal metabolite spectrogram can fully reflect comprehensive influence in aspects such as nutrition, individual and environment and timely and sensitively reflect correlation among animal spermine nutrition and metabolism conditions thereof as well as intestinal development. According to the method, biological markers related to the intestinal spermine nutrition process can be screened, the intrinsic quality of the spermine nutrition process is disclosed, the method has important theory and practice significance for perfecting digestive absorption functions of the animals, optimizing the feed formula and improving healthy growth of the animals, and the gaps that the overall nutrition metabolism conditions of animal intestinal development cannot be evaluated by combining with the metabonomics and the animal intestinal development cannot be improved are filled. Moreover, the research result proves that the method has actual application values in breeding industry.

Description

technical field [0001] The invention belongs to the technical field of animal nutrition and metabolism evaluation, and in particular relates to a metabonomics test method for spermine regulating intestinal development of mammals. Background technique [0002] The main purpose of animal nutrition research is to reveal the essence of the nutritional process and to clarify the dynamic quantitative change law in the process of nutrient metabolism and transformation. Nutritional metabolism is always at the core of nutritional science research. Genetic information is transmitted from genes to functional proteins through transcripts, and finally reflected by metabolites. Metabolism is the result of the comprehensive influence of various factors in the body's internal and external environments, and involves the effects of various substances. The life performance or biological characteristics in which multiple substances participate are not only intertwined or occur in parallel, bu...

Claims

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Application Information

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IPC IPC(8): G01N24/08
Inventor 刘光芒贾刚陈小玲赵华汪静
Owner SICHUAN AGRI UNIV
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