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Application of lamprey CRBGP in preparation of drug for resisting malignant glioma

A technology for malignant glioma and lamprey, which is applied in the direction of anti-tumor drugs, drug combinations, and pharmaceutical formulations, and can solve the problems of anti-glioblastoma drugs that have not yet been seen

Inactive Publication Date: 2017-09-08
LIAONING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there has been no report on the application of lamprey CRBGP as a sodium ion channel blocker in the preparation of anti-glioblastoma drugs so far

Method used

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  • Application of lamprey CRBGP in preparation of drug for resisting malignant glioma
  • Application of lamprey CRBGP in preparation of drug for resisting malignant glioma
  • Application of lamprey CRBGP in preparation of drug for resisting malignant glioma

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Experimental program
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Effect test

Embodiment Construction

[0067] 1. Purification of native CRBGP

[0068] The lampreys were sacrificed, their oral glands were stripped off, and the liquid in the oral glands was sucked out with a syringe at -80°C for storage. The extracted liquid was diluted to 24 mg / ml with a buffer solution containing 50 mM Tris-HCl and 50 mM NaCl (pH 7.4), and then centrifuged at 10,000 rpm for 10 min at 4°C to obtain the supernatant. About 380 μl of supernatant was added to a 1-meter-long Sephadex G-75 chromatography column, the sample flow rate was 700 μl / min, and about 1.2 ml of eluate was collected in each Eppendor tube. The purity of the protein was detected by 12% polyacrylamide gel electrophoresis (SDS-PAGE), and the concentration of the protein was determined by the BCA method.

[0069] 2. Expression and purification of recombinant CRBGP

[0070] (1) The CRBGP gene with the signal peptide removed and the vector pEGX-4T-1 were double-enzymatically digested, ligated overnight at 16°C, and the ligated prod...

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Abstract

The invention discloses application of a lamprey CRBGP in the preparation of a drug for resisting malignant glioma. The application finds that natural and recombinant lamprey CRBGPs are capable of effectively inhibiting processes of multiplication, adhesion, migration, infiltration and the like of U251 cells of the malignant glioma and present a dose-dependent property. Therefore, the lamprey CRBGP can be used as a sodium ion channel blocking agent in the application of the preparation of the drug for resisting the malignant glioma and has the advantages of low toxicity, high efficiency and the like.

Description

technical field [0001] The invention relates to a new application of lamprey CRBGP, in particular to an application of lamprey CRBGP in the preparation of anti-malignant glioma drugs. Background technique [0002] Cysteine ​​rich secretory proteins (CRISP) are a class of proteins that are highly conserved in evolution, containing 16 highly conserved cysteine ​​residues that can form 8 pairs of disulfide bonds. The spatial structure is mainly composed of three parts: the N-terminal PR-1 domain (pathogenesis-related group 1 domain), the C-terminal CRD domain (cysteine-rich domain), and the hinge region connecting these two domains. In recent years, studies have found that members of the CRISP family in mammals are mainly distributed in the reproductive tract, and play a very important role in sperm maturation, sperm-egg fusion, and the immune system; members of the CRISP family in non-mammals are mainly distributed in In the secretion of glands, it can block K + , Ca 2+ And...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P35/00
CPCA61K38/1706
Inventor 肖蓉韩健美
Owner LIAONING NORMAL UNIVERSITY
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