Phascolosoma esculenta artificial spawning induction method
A technology for artificial induction of labor and sclerozoites, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of difficulty in obtaining better induction of labor, high deformity rate of fertilized eggs, and low survival rate of parental bodies, and achieves increased production. The effect of large parent utilization rate, improving survival rate, and avoiding a large number of deformed fertilized eggs
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Embodiment 1
[0023] On August 10, 2015, artificially induced labor was carried out on the wild Derma spp. parents collected from the mangrove forest in the first work area of the Beihai Zhulin Salt Field. The specific implementation steps are as follows:
[0024] (1) Preparation of the oxytocin pool:
[0025] The oxytocin pool is an indoor cement pool with a size of 5.3 x 3.5 x 1.2 meters. A layer of net curtain with a light transmittance of 50% is drawn above the oxytocin pool to avoid direct sunlight. The air stones are evenly distributed in the pool to keep the dissolved oxygen in the seawater above 5mg / L. Prepare a mesh basket with a specification of 1.2×0.6×0.2 meters, with a mesh size of 60 meshes, so that unfertilized eggs and fertilized eggs can follow the water flow through the mesh basket and spread all over the pool without allowing the parent body to drill into the mesh stuck, resulting in the death of the parent.
[0026] (2) Select parent:
[0027] The body weight of th...
Embodiment 2
[0037] On July 16, 2016, the wild broodstock of the Xiajiabang mangrove forest in Jiangping Town, Fangchenggang was collected, and artificial induction was carried out. The specific implementation steps are as follows:
[0038] (1) Preparation of the oxytocin pool:
[0039] The oxytocin pool is an indoor cement pool with a size of 5.3 x 3.5 x 1.2 meters. A layer of net curtain with a light transmittance of 75% is drawn above the oxytocin pool to avoid direct sunlight. The air stones are evenly distributed in the pool to keep the dissolved oxygen in the seawater above 5mg / L. Prepare a mesh basket with a size of 1.2×0.6×0.2 meters and a mesh size of 150 meshes, so that unfertilized eggs and fertilized eggs can follow the water flow through the mesh basket and spread all over the pool without allowing the parent body to drill into the mesh stuck, resulting in the death of the parent.
[0040] (2) Select parent:
[0041] The body weight of the parents was 1.8-6.5g, with an avera...
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