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Functional marker AS-ALS for anti-imazethapyr herbicide genotype detection and application of functional marker AS-ALS

An AS-ALS-F1, genotype technology, applied in the direction of DNA/RNA fragment, recombinant DNA technology, microbial determination/inspection, etc., can solve the problem of cumbersome herbicide resistance gene detection process, etc., and achieve accurate and reliable amplification products. , strong practical value, simple operation effect

Inactive Publication Date: 2017-10-20
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

[0007] The object of the present invention is to provide a molecular marker AS-ALS for detecting herbicide-resistant or herbicide-sensitivity genes in samples to solve the problem of cumbersome detection of herbicide-resistant genes;

Method used

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  • Functional marker AS-ALS for anti-imazethapyr herbicide genotype detection and application of functional marker AS-ALS
  • Functional marker AS-ALS for anti-imazethapyr herbicide genotype detection and application of functional marker AS-ALS
  • Functional marker AS-ALS for anti-imazethapyr herbicide genotype detection and application of functional marker AS-ALS

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[0041] Below in conjunction with accompanying drawing description, technical method of the present invention is described with specific embodiment:

[0042] (The embodiments of the present invention are described in detail below, wherein the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions. The embodiments described below by referring to the drawings are only used to explain the present invention, and cannot understood as a limitation of the invention.)

[0043] The inventor's team screened out an imazethapyr-resistant rice material "Jinjing 818" from 7403 rice variety resource materials through extensive research. Sequencing and analysis of the ALS gene sequence of "Jinjing 818" revealed that there is a mutation in the 1880th position of the coding region of the ALS gene, where a base G is converted to A.

[0044] The gene can be transferred to cultivars through backcrossing, but traditionally, it needs ...

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Abstract

The invention discloses a functional marker AS-ALS for anti-imazethapyr herbicide genotype detection and an application of the functional marker AS-ALS. The marker comprises a primer combination F1N (AS-ALS-F1 and AS-ALSn-R2-2) and a primer combination F1M (AS-ALS-F1 and AS-ALSm-R2-1), the F1N can amplify a stripe sample, namely, an ALS-G genotype, the F1M can amplify a stripe sample, namely, an ALS-A genotype, both the F1N and the F1M can amplify a stripe sample, and namely, a heterozygous genotype of the ALS-G and the ALS-A. The marker and a method can be used for genotype detection of variety resources and filial generation and are beneficial to accelerating an herbicide-resistant breeding progress. The method is high in accuracy and detection efficiency, simple to operate and low in cost and has a high practical value.

Description

technical field [0001] The invention belongs to the field of agricultural bioengineering, and relates to a functional marker AS-ALS used for genotype detection of imazethapyr herbicide resistance and application thereof. Background technique [0002] Rice is the staple food for nearly half of the world's population, and it is also one of the main food crops in my country. The traditional rice cultivation method is mainly manual cultivation, which is time-consuming and labor-intensive. With the increase of labor costs, the cost of rice cultivation has also increased significantly, and the benefits are low. In recent years, with the development of my country's new urbanization and modern agriculture, rice production is developing towards intensification, scale, and mechanization, and light and simple cultivation such as machine transplanting and direct seeding has become a development trend. However, weeds and weedy rice are easy to breed in direct-directed rice fields, which...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 杨杰王芳权范方军王军李文奇许扬仲维功杨睿
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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