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Method of increasing adipose cell transplantation survival rate

An adipocyte and adipose stem cell technology, applied in the field of improving the survival rate of adipocyte transplantation, can solve the problems of low survival rate of adipocytes and cumbersome steps, etc.

Inactive Publication Date: 2017-10-24
溯源生命科技股份有限公司
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AI Technical Summary

Problems solved by technology

[0008] The survival rate of the existing adipocyte culture process is low, and the steps are cumbersome, so it is necessary to study a method that can improve the survival rate of adipocyte transplantation

Method used

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Embodiment Construction

[0011] This specific embodiment adopts the following technical scheme: the method for improving the survival rate of adipocyte transplantation is as follows: step 1, adipose stem cell acquisition, 1-1, put the adipose tissue derived from liposuction into a pre-cooled container containing 1% double antibody In normal saline, sealed, under low temperature conditions, and sent back to the laboratory at 2-8°C; 1-2. Rinse the above adipose tissue with normal saline to remove residual blood cells and tissue fragments, put the adipose tissue into a centrifuge tube, Record the original volume, add an equal volume of digestive solution to the tissue, the digestive solution is prepared by mixing 0.25% trypsin and 0.1% type I collagenase at a ratio of 1:1, and put it in a 37°C air bath shaker The digested tissue was digested for 30 minutes, and then the digested tissue was left to stand for 5 minutes. At this time, the liquid surface was divided into 3 layers. The upper layer of yellow oi...

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Abstract

The invention discloses a method of increasing adipose cell transplantation survival rate and relates to the technical field of cell transplantation. The method of increasing adipose cell transplantation survival rate comprises the following steps: S1, acquiring adipose-derived stem cells; and S2, acquiring and transplanting adipose cells and harvesting and counting cultured adipose-derived stem cells while treating the adipose cells; mixing the adipose-derived stem cells with the adipose cells in a proportion of 2: 1; and transplanting obtained cell suspension to a corresponding part. The acquiring step of the adipose-derived stem cells is relatively simple, and the adipose-derived stem cells can secrete various growth nutritional factors to improve the micro-environment in tissues and organs, so that death caused by lack of nutrients after the adipose cells are filled can be greatly improved without doubt by co-transplantation of autologous adipose-derived stem cells and adipose cells, and therefore, the transplantation survival rate of the adipose cells is increased.

Description

technical field [0001] The invention relates to a method for improving the survival rate of fat cell transplantation, which belongs to the technical field of cell transplantation. Background technique [0002] With age, the skin will gradually appear aging phenomena such as relaxation, weakening of elasticity, and local depression. Plastic surgery often extracts excess body fat from patients, purifies the fat cells and replenishes them to the parts that need to be filled. Fat damage is minimized. Generally, after low-speed centrifugation, purification, and purification treatment, complete fat cell particles are selected, and fine joint injection technology is used to perform multi-level and multi-point facial filling / wrinkle removal / Shaping etc. [0003] Autologous fat transplantation has a history of hundreds of years. It is easy to be accepted by patients because of its rich source, easy material acquisition, simple operation, good filling shape, small local reaction, n...

Claims

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0653C12N5/0667C12N2509/00
Inventor 周丽英汪晓敏袁卫平万谦
Owner 溯源生命科技股份有限公司
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