Drug for preventing and controlling plant greensickness
A Verticillium wilt and medicament technology, applied in the field of medicaments for the prevention and treatment of plant Verticillium wilt, can solve problems such as unsatisfactory plant Verticillium wilt effects, achieve good control effects and broad application prospects
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Examples
Embodiment 1
[0025] 1. After the cotton seeds are develored according to the conventional method, they are planted in the seedling pots of the greenhouse under the conditions of long sunshine, 30°C during the day and 20°C at night. During the growth period of cotton, water and moisturize in moderation.
[0026] 2. After the cotton seedling grows the first true leaf, use a sharp object to damage the cotton root, inoculate Verticillium dahliae in upland cotton Xu 42, and the inoculated strain is Linxi 2, a strong pathogenic strain of Verticillium dahliae -1 spore suspension at a concentration of 5×10 6 cfu / mL, the inoculum volume is 5mL / plant, and no less than 24 cotton plants are inoculated.
[0027] 3. After the inoculation was completed one day, spray the molar concentration of 10nM, 100nM and 1000nM by distilled water on the cotton seedlings that had been inoculated, and spray the 24-epibrassinolide (eBL) solution until all the leaves have solution (per A cotton seedling is about 2-3mL...
Embodiment 2
[0031] 1. After the cotton seeds of different varieties are develored according to the conventional method, they are planted in the seedling pots of the greenhouse. The growth conditions are long sunshine, 30°C during the day and 20°C at night. During the growth period of cotton, water and moisturize in moderation.
[0032] 2. After the cotton seedlings grow the first true leaves, use a sharp object to damage the roots of the cotton, and inoculate different cotton varieties with Verticillium dahliae. The inoculated strain is Linxi 2, a strong pathogenic strain of Verticillium dahliae -1 spore suspension at a concentration of 5×10 6 cfu / mL, the inoculum volume is 5mL / plant, and the inoculated plants of each cotton variety are not less than 24.
[0033] 3. One day after the inoculation is completed, start to spray the eBL solution with a molar concentration of 100nM prepared by distilled water on the cotton seedlings that have been inoculated until all leaves have the solution ...
Embodiment 3
[0037] 1. After the cotton seeds are develored according to the conventional method, they are planted in the seedling pots of the greenhouse under the conditions of long sunshine, 30°C during the day and 20°C at night. During the growth period of cotton, water and moisturize in moderation.
[0038] 2. After the cotton seedling grows the first true leaf, use a sharp object to damage the cotton root, inoculate Verticillium dahliae with Xuzhou 142, a strong pathogenic strain of Verticillium dahliae Quzhou 1 -1, the spore suspension of medium pathogenic bacterial strain Nangong 4-2 and weak pathogenic bacterial strain Pingxiang 5-2, the concentration is 5×10 6 cfu / mL, the inoculum volume is 5mL / strain, and each strain inoculates no less than 24 cotton plants.
[0039] 3. One day after the inoculation is completed, start to spray the eBL solution with a molar concentration of 100nM prepared by distilled water on the cotton seedlings that have been inoculated until all leaves have ...
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Description
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Application Information
- IPC
- A01N43/22; A01P3/00
- CPC
- A01N43/22
- Inventors
- 朱生伟; 蒙福宁