Drug for preventing and controlling plant greensickness

A Verticillium wilt and medicament technology, applied in the field of medicaments for the prevention and treatment of plant Verticillium wilt, can solve problems such as unsatisfactory plant Verticillium wilt effects, achieve good control effects and broad application prospects

Inactive Publication Date: 2017-10-31
INST OF BOTANY CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a medicament for preventing and treating Verticillium wilt in plants, especially for preventing and treating Verticillium wilt in cotton. The medicament provided by the invention can effectively prevent and treat Verticillium wilt in plants, and overcomes the existing effects of existing chemical agents in preventing and treating Verticillium wilt in plants. suboptimal problem

Method used

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  • Drug for preventing and controlling plant greensickness
  • Drug for preventing and controlling plant greensickness
  • Drug for preventing and controlling plant greensickness

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. After the cotton seeds are develored according to the conventional method, they are planted in the seedling pots of the greenhouse under the conditions of long sunshine, 30°C during the day and 20°C at night. During the growth period of cotton, water and moisturize in moderation.

[0026] 2. After the cotton seedling grows the first true leaf, use a sharp object to damage the cotton root, inoculate Verticillium dahliae in upland cotton Xu 42, and the inoculated strain is Linxi 2, a strong pathogenic strain of Verticillium dahliae -1 spore suspension at a concentration of 5×10 6 cfu / mL, the inoculum volume is 5mL / plant, and no less than 24 cotton plants are inoculated.

[0027] 3. After the inoculation was completed one day, spray the molar concentration of 10nM, 100nM and 1000nM by distilled water on the cotton seedlings that had been inoculated, and spray the 24-epibrassinolide (eBL) solution until all the leaves have solution (per A cotton seedling is about 2-3mL...

Embodiment 2

[0031] 1. After the cotton seeds of different varieties are develored according to the conventional method, they are planted in the seedling pots of the greenhouse. The growth conditions are long sunshine, 30°C during the day and 20°C at night. During the growth period of cotton, water and moisturize in moderation.

[0032] 2. After the cotton seedlings grow the first true leaves, use a sharp object to damage the roots of the cotton, and inoculate different cotton varieties with Verticillium dahliae. The inoculated strain is Linxi 2, a strong pathogenic strain of Verticillium dahliae -1 spore suspension at a concentration of 5×10 6 cfu / mL, the inoculum volume is 5mL / plant, and the inoculated plants of each cotton variety are not less than 24.

[0033] 3. One day after the inoculation is completed, start to spray the eBL solution with a molar concentration of 100nM prepared by distilled water on the cotton seedlings that have been inoculated until all leaves have the solution ...

Embodiment 3

[0037] 1. After the cotton seeds are develored according to the conventional method, they are planted in the seedling pots of the greenhouse under the conditions of long sunshine, 30°C during the day and 20°C at night. During the growth period of cotton, water and moisturize in moderation.

[0038] 2. After the cotton seedling grows the first true leaf, use a sharp object to damage the cotton root, inoculate Verticillium dahliae with Xuzhou 142, a strong pathogenic strain of Verticillium dahliae Quzhou 1 -1, the spore suspension of medium pathogenic bacterial strain Nangong 4-2 and weak pathogenic bacterial strain Pingxiang 5-2, the concentration is 5×10 6 cfu / mL, the inoculum volume is 5mL / strain, and each strain inoculates no less than 24 cotton plants.

[0039] 3. One day after the inoculation is completed, start to spray the eBL solution with a molar concentration of 100nM prepared by distilled water on the cotton seedlings that have been inoculated until all leaves have ...

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Abstract

The invention discloses a drug for preventing and controlling plant greensickness. The drug is a solution of 24-epibrassinolide or derivative thereof, preferably an aqueous solution. In the aqueous solution, the molar concentration of 24-epibrassinolide or derivative thereof is 1nM-1000nM. The plant greensickness is caused by Verticillium dahliae. The application of 4-epibrassinolide or derivative thereof in preparing Verticillium dahliae bactericide or preventing and controlling the plant greensickness also belongs to the protection range of the invention. The bactericide or drug provided by the invention has excellent preventing and controlling effects for the greensickness of different kinds of cottons, and meanwhile, the bactericide or drug has excellent preventing and controlling effects for the greensickness caused by different greensickness bacterial strains (such as, strong greensickness pathogenic bacteria Linxi 2-1 and temporal hairline 1-1, medium pathogenic bacteria strain Nangong 4-2 and weak pathogenic bacteria strain Pingxiang 5-2). Thus, the bactericide or drug provided by the invention can effectively prevent and control the plant greensickness and has wide application prospect.

Description

technical field [0001] The invention relates to a medicament for preventing and treating plant verticillium wilt, belonging to the technical field of pesticides. Background technique [0002] Cotton verticillium wilt is one of the important diseases that endanger cotton production at present. It is called the "cancer" of cotton and spreads all over the cotton-producing areas in the world. Its pathogen is Verticillium dahliae. Cotton verticillium wilt is a soil-borne fungal disease that infects along the vascular system, which seriously endangers the growth and development of cotton, leads to a reduction in cotton production, causes huge economic losses to cotton production, and seriously affects the sustainable development of the cotton industry. Because the pathogenic mechanism of the pathogen and the genetic mode of cotton resistance to Verticillium wilt are still relatively poorly understood, the current prevention and treatment of cotton Verticillium wilt is mainly to br...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/22A01P3/00
CPCA01N43/22
Inventor 朱生伟蒙福宁吴金霞张治国罗小敏
Owner INST OF BOTANY CHINESE ACAD OF SCI
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