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A method for obtaining hybrids of wild species of Melon and cultivated thick-skinned melon

A technology of thick-skinned melons and wild species, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve difficult problems such as success, and achieve the effect of broadening the genetic basis

Active Publication Date: 2019-05-07
南京利华农业科技有限公司
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  • Application Information

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Problems solved by technology

The use of wild species of the genus Melon to improve the resistance of cultivated melons is one of the most effective methods to increase the resistance of melons. However, due to factors such as hybrid reproductive barriers, it is difficult to successfully hybridize wild species of the genus Melon with cultivated melons.

Method used

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  • A method for obtaining hybrids of wild species of Melon and cultivated thick-skinned melon
  • A method for obtaining hybrids of wild species of Melon and cultivated thick-skinned melon
  • A method for obtaining hybrids of wild species of Melon and cultivated thick-skinned melon

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Embodiment 1

[0020] The method provided by the invention to obtain the wild species of Melon and cultivated thick-skinned melon hybrids, its implementation is as follows:

[0021] Melon inbred line 'MHY07' (from Zhenjiang Ruifan Agronomy Co., Ltd.) was used as the female parent, and 'Mapaogua' (from Zhenjiang Ruifan Agronomy Co., Ltd.) was used as the male parent for artificial crossing. Bagging, artificial pollination is carried out on sunny days, and pollination is carried out on the day when the flowers open. Before pollination, use clean tweezers to gently remove the pollen blocks of the male parent, place them in a dry beaker or on paper, and place them on the stigma of the female parent, and bag them after pollination. Take the hybrid inflorescence within 15-21 days after pollination, gently peel off the ovary, and then wash the ovary with running water for 10 minutes, then soak it with 0.4% NaCl for 30 minutes, then wash it with water for 5-6 times, and then rinse it with 2% NaCl S...

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Abstract

Belonging to the technical field of plant breeding, the invention relates to a method for acquiring cucumis melo wild species and cultivating thick skin cucumis melo hybrid species. The main technical measures include: conducting cucumis melo interspecific hybridization, then conducting aseptic treatment on an ovary pollinated for 15-21d, taking out immature embryos, then putting the immature embryos in a hormone added 1 / 2MS medium for culture, then carrying out subculture to obtain seedlings, controlling the culture temperature at 26DEG C, the daily illumination time at 10-12h, and the illuminance at 1600-2000lx. The method provided by the invention can solve the key technical problem of cucumis melo interspecific hybridization reproductive disorder, and also can acquire cucumis melo interspecific hybrid seedlings.

Description

technical field [0001] The invention relates to a method for obtaining hybrids of wild melons and cultivated thick-skinned melons, and belongs to the technical field of plant breeding. Background technique [0002] Melon (Cucumis melo L.) is an annual herbaceous plant belonging to Cucurbitaceae Melon, which is widely cultivated at home and abroad. Due to the long-term neglect of the collection, preservation and utilization of muskmelon resistance resources, the melon germplasm resources are relatively single, the genetic differences between varieties are small, and the genetic background of the germplasm resources is narrow. Moreover, with the aggravation of heavy cropping, the diseases of melons have advanced and aggravated. Among them, powdery mildew and creeping blight have become the main diseases of muskmelon production in my country, especially in the high-humidity and rainy eastern coastal areas. Cultivation with devastating consequences. Improving the resistance of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/001A01H4/008
Inventor 孙春青马志虎戴忠良潘跃平毛忠良张振超吴国平秦文斌姚悦梅潘永飞
Owner 南京利华农业科技有限公司
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