Drug target and applications thereof related to castration-resistant prostate cancer

A technology for prostate cancer and castration resistance, which is used in antitumor drugs, drug combinations, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2017-11-21
SHANGHAI CHANGHAI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research and clinical significance of cellular senescence and its related secreted proteins in solid tumors, and the mechanism of cellular senescence and its related secreted proteins in castration-resistant prostate cancer have not yet been seen. to report

Method used

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  • Drug target and applications thereof related to castration-resistant prostate cancer
  • Drug target and applications thereof related to castration-resistant prostate cancer
  • Drug target and applications thereof related to castration-resistant prostate cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The specific implementation manners provided by the present invention will be described in detail below.

[0023] 1. Main experimental materials:

[0024] MDV3100: Selleck Corporation

[0025] DMSO: Sigma Corporation

[0026] Prostate cancer LNCaP FGC cells: introduced by ATCC

[0027] 1640 medium, fetal bovine serum FBS: Gibco

[0028] SA-β-gal staining kit: CST company

[0029] Protein chip: Huaying Biotechnology Co., Ltd.

[0030] 2. Method

[0031] 2.1 Cell culture and administration

[0032] The prostate cancer LNCaP FGC cell line was introduced from ATCC, and the cells were cultured in RMPI 1640 medium (Gibco) at 25 cm 2 In a high-adhesion culture flask (Corning), the medium was supplemented with 10% FBS (Gibco) and 1% penicillin-streptomycin (Gibco), and the culture condition was 5% CO 2 , 37°C, after the cell density reaches about 70%-80%, conduct subculture to ensure that the cell density will not be too large and affect the cell state. Digest with con...

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Abstract

The invention relates to applications of antagonist of aging related secreted factors, and a pharmaceutical composition for inhibiting castration-resistant prostate cancer. The aging related secreted factors are selected from MIP-3-alpha, RANTES, MCP-2 factors. The invention also provides applications of the aging related secreted factors to preparation of products for predicting prostate cancer prognosis, and the aging related secreted factors are selected from MIP-3-alpha, RANTES, and MCP-2 factors. The composition is advantageous in that based on discovery that MDV3100 can induce LNCaP FGC cells to generate cell aging, protein chips are employed to discover that MDV3100 can induce the LNCaP FGC cells to secrete a lot of chemotactic factors, such as MIP-3-alpha, RANTES, MCP-2 factors and the like, as well as SASP proteins, such as cytokine and the like, and a base is provided for subsequent researches, such as SASP and CRPC, prostate carcinoma with bone metastasis and the like.

Description

technical field [0001] The present invention relates to the technical field of tumor-related drug targets, in particular, relates to a castration-resistant prostate cancer-related drug target and its application. Background technique [0002] Prostate cancer is the most common malignant tumor of the male reproductive system, and its incidence increases with age. It ranks third in male cancer incidence and sixth in mortality in the world, and ranks first in male cancer incidence in the United States. The second place in the case fatality rate. In recent years, the incidence of prostate cancer in Chinese men has continued to rise, posing a serious threat to men's health and bringing enormous pressure to society. Androgen deprivation therapy (ADT) is the main treatment for advanced prostate cancer, most of the patients have a good response, but with the continuous use of androgen antagonists, most of the patients have androgen antagonism The performance of drug resistance, ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/19A61P39/06A61P35/00
Inventor 孙颖浩王野施敏凤
Owner SHANGHAI CHANGHAI HOSPITAL
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