Reagent for homogenizing sputum microorganism specimen

A sputum and specimen technology, which is applied in the field of sputum microorganism specimen homogenization reagents, can solve the problems of short storage time of reagents, poor stability of dithiothreitol components, lack of ability and the like

Inactive Publication Date: 2017-11-28
李齐 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although the above-mentioned sputum digestive juices and digestion methods have been used for the detection of sputum samples, the results of clinical practice show that these digestive juices can homogenize and protect the bacteria in the...

Method used

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  • Reagent for homogenizing sputum microorganism specimen
  • Reagent for homogenizing sputum microorganism specimen
  • Reagent for homogenizing sputum microorganism specimen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1 Preparation and use of sputum digestive juice of the present invention and contrast sputum digestive juice

[0014] Preparation of sputum digestive juice of the present invention

[0015] Prepare the phlegm digestive juice of the present invention according to the following list of ingredients:

[0016] All reagents are of domestic analytical grade, from Sinopharm Group, Xusheng Biology, etc.

[0017]

[0018] The prepared sputum digestion solution was divided into 15ml / parts and stored in a refrigerator at 4°C. When in use, add 50ml of sterile water to each serving to make a working solution. Add roughly the same amount of working solution to the sputum, digest in a water bath at 37°C for 15 minutes, centrifuge at 10,000 rpm / min for 5 minutes, and then take the precipitate for bacterial culture or DNA extraction.

Embodiment 2

[0019] Embodiment 2 compares with existing sputum digestive juice digestion effect

[0020] Commercially available Sputasol (Liquid) SR0233 (OXOID), use according to the instructions.

[0021] Digestive liquid 1: the sputum digestive liquid in Example 1 of Chinese Patent Application No. 201110126201.2, the method of use is as described in the specification of the application.

[0022] Digestive juice 2: the sputum digestive juice in Example 1 of Chinese Patent Application No. 201510031792.3, the method of use is as described in the specification of the application.

[0023] 2.1 Homogenization effect

[0024] Take 1 sample of sputum suction (volume about 40ml), divide it into 4 equal parts, and wash 3 times with 200ml sterile saline respectively to remove normal respiratory flora on the surface of sputum. Sputum was digested according to the method described in Example 1 and Sputasol (Liquid) SR0233 (OXOID), Chinese Patent Application No. 201110126201.2, and Chinese Patent Ap...

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Abstract

The invention provides a method and a reagent for homogenizing a sputum specimen. The reagent is prepared from 0.2% (w/v) of N-acetyl-L-cysteine, 0.1% (w/v) of trypsin, 0.4% (w/v) of triethanolamine, 0.2% (w/v) of potassium chloride, 1.5% (w/v) of monopotassium phosphate, 0.2% (w/v) of dipotassium hydrogen phosphate and a sterile water solvent.

Description

technical field [0001] The invention relates to a method for homogenizing sputum samples and a reagent used for treating clinical sputum samples, which can effectively homogenize sputum and protect bacteria including Legionella in the sputum from being damaged , which is conducive to further analysis of sputum samples. Background technique [0002] Sputum samples collected by natural expectoration method and sputum suction method in the examination of respiratory diseases have obvious heterogeneity, and the distribution of bacteria and other components is very uneven. It is generally necessary to use sputum digestive juice to homogenize the samples . With the improvement of inspection accuracy and experimental quality control requirements, more and more attention has been paid to the pretreatment of specimens. According to daily work observations, the culture results of sputum specimens and the rate of agreement with actual clinical conditions are still not ideal, and sputu...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/01
CPCC12Q1/04
Inventor 李齐陈泓达
Owner 李齐
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