A kind of living garbage degrading compound bacteria and its application

A technology of domestic waste and compound bacteria, applied in the direction of bacteria, fungi, microorganisms, etc., can solve the problems of poor biochemical treatment effect of domestic waste, and achieve the effect of safe and convenient use and high waste reduction rate.

Active Publication Date: 2020-05-19
ZHEJIANG HUAQINGYUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings of the poor biochemical treatment effect of domestic waste in the prior art, a compound microbial preparation capable of mass production and stable performance that efficiently degrades domestic waste is provided

Method used

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  • A kind of living garbage degrading compound bacteria and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Strain screening and identification:

[0027] (1) Strain screening: Selective medium is used to screen the strains in domestic waste reactors;

[0028] (2) Re-screening of bacterial strains: screening of high-efficiency bacteria for the single strains screened;

[0029] (3) Strain enrichment: liquid enrichment culture for high-efficiency strains;

[0030] ⑷Construction of composite strains: combine and test the strains;

[0031] ⑸ Identification of strains in complex bacteria: Molecular biological identification of the strains.

[0032] Wherein, step (1) described strain screening, the present invention provides following method:

[0033] Use a sterile pipette gun to pipette 10.0mL of the biochemical system mixed solution of the domestic waste disposer into a conical flask filled with 90.0mL of sterile water, and mix well. Then take 1.0mL of the diluted solution in a test tube filled with 9.0mL sterile water (-10 -2 ), then use a sterile pipette to pipette 1.0mL fr...

Embodiment 2

[0066] Comparison of degradation efficiencies of different formulations of composite bacterial agents and single bacterial agents

[0067] The three screened strains of Bacillus amyloliquefaciens HQY-01, Bacillus HQY-04, and Candida spp. HQY-06 were respectively fermented and cultured: inoculated into the nutrient solution, at 35°C, with an oscillation frequency of 120r / min for 4 days.

[0068] The nutrient solution contains the following components (containing per volume of the nutrient solution): peptone 10g / L, sodium chloride 5g / L, yeast extract 10g / L, glucose 10g / L, and the pH is adjusted to 7.

[0069] The number of viable bacteria in the culture medium of Bacillus amyloliquefaciens HQY-01, Bacillus HQY-04, and Candida phalangis HQY-06 obtained by fermentation is 2.5*10 7 CFU / mL, 2.5*10 7 CFU / mL, 4.5*10 7 CFU / mL

[0070] Take 6 parts of 100mL of domestic waste culture solution, add 1*10 per 100mL 7 The number of CFU was added to the Bacillus amyloliquefaciens HQY-01...

Embodiment 3

[0080] Embodiment 3 composite bacteria preparation is used for the degradation of house refuse:

[0081] 1. Fermentation of compound bacterial preparation

[0082] Fermentation includes the following steps: Inoculate the three screened strains: Bacillus amyloliquefaciens HQY-01, Bacillus bacillus HQY-04, and Candida albicans HQY-06 into the nutrient solution respectively, at 35°C, the oscillation frequency Cultivate for 4 days under the condition of 120r / min.

[0083] The nutrient solution contains the following components (containing per volume of the nutrient solution): 10 g / L of peptone, 5 g / L of sodium chloride, 10 g / L of yeast extract, 10 g / L of glucose, and the pH is adjusted to 7.

[0084]Gained Bacillus amyloliquefaciens HQY-01, Bacillus HQY-04, and the number of live bacteria in the culture solution of Candida phalangis HQY-06 are respectively 2.5*10 7 CFU / mL, 2.5*10 7 CFU / mL, 4.5*10 7 CFU / mL

[0085] According to the ratio of the effective number of viable bacte...

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Abstract

The invention discloses household garbage degrading compound bacteria, which is composed of Bacillus amyloliquefaciens HQY-01, Bacillus sp HQY-04 and Candida pseudolambica HQY-06 and was preserved in the China Center for Type Culture Collection, Wuhan, China on Feb. 16, 2017 with the preservation numbers respectively being CCTCC NO: M2017040, CCTCC NO: M2017042 and CCTCC NO: M2017043. The household garbage degrading compound bacteria can be used for degrading household garbage. Waste volume reduction rate is high, and COD, ammonia nitrogen and total nitrogen removal rate are high.

Description

technical field [0001] The invention relates to a biological agent in a domestic waste treatment process, and in particular provides a domestic waste-degrading composite bacterium and its application. Background technique [0002] At present, the treatment methods of domestic waste in my country are mainly biological methods. Due to the characteristics of high organic matter content and complex components of domestic waste, ordinary biological treatment methods are less effective. Therefore, it is of great significance to study the composition of microorganisms in the biochemical treatment system of domestic waste, to screen out strains of efficient degradation of domestic waste, to understand the characteristics of each strain, and to cultivate a compound bacterial agent that can efficiently degrade domestic waste, which is of great significance for optimal treatment. [0003] Composite microbial agents refer to microbial agents made from two or more microbial strains that ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16B09B3/00C12R1/07C12R1/72
CPCB09B3/00C12N1/16C12N1/20
Inventor 朱勤华于通戴军张文涛朱慧强李健
Owner ZHEJIANG HUAQINGYUAN BIOTECH CO LTD
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