Micro-fluidic chip and micro-fluidic chip based analyzing device and method

A microfluidic chip and analysis device technology, applied in the direction of measuring devices, analysis materials, chemical instruments and methods, etc., can solve the problems of low purity and recovery, difficult to remove polymers, and many foreign proteins, etc., and achieve simple and convenient operation Effect

Active Publication Date: 2017-12-08
SHENZHEN HUIXIN LIFE TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are many problems in using PEG to precipitate exosomes: such as low purity and recovery rate, more impurities, uneven particle size, and polymers that are difficult to remove. In addition, the physical effects used in PEG-base such as mechanical force or Tween- Chemical additives such as 20 will destroy exosomes, etc., so it is easy to be questioned
For exosomes, which are much smaller in size than cells, the efficiency and sensitivity of flow cytometry will be greatly reduced

Method used

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  • Micro-fluidic chip and micro-fluidic chip based analyzing device and method
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  • Micro-fluidic chip and micro-fluidic chip based analyzing device and method

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Embodiment Construction

[0033] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, the specific implementation manners of the present invention will now be described in detail with reference to the accompanying drawings.

[0034] A tumor as used in the present invention is defined as a mass of abnormally growing cells. Tumors useful for the present invention include, but are not limited to, prostate cancer, breast cancer, lung cancer, colon cancer, gastric cancer, endometrial cancer, liver cancer, esophageal cancer, bladder cancer, oral cancer, thyroid cancer, pancreatic cancer, retinal and skin cancer, preferably for pancreatic cancer.

[0035] "Tumor sample" refers to a sample taken from a patient before or after resection of the tumor.

[0036] In the present invention, "liquid sample" is called "tumor sample", and "liquid sample" can be isolated from any tissue, cell, cell extract, saliva, urine, serum, whole blood, plasma concentrat...

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Abstract

The invention relates to a micro-fluidic chip. The micro-fluidic chip is provided with a micro-fluidic channel, a filtering element and a detection area, wherein the detection area is arranged in the micro-fluidic channel and used for filtering a fluid sample to enable the fluid containing target particles to flow into the detection area. The invention also relates to a micro-fluidic chip based analyzing device. The device comprises the micro-fluidic chip and a detection analyzing unit; the detection analyzing unit is used for detecting and analyzing the detection area to obtain biological information of the target particles. In addition, the invention relates to a method for analyzing the target particles in the fluid sample.

Description

technical field [0001] The invention relates to a microfluidic chip and its application, belonging to the field of biological detection. Background technique [0002] Biological samples, such as exosomes, can carry a variety of proteins, mRNA, and miRNA, and participate in processes such as cell communication, cell migration, angiogenesis, and tumor cell growth. At present, the detection methods of exosomes mainly include enzyme-linked immunosorbent assay (ELISA) and flow cytometry. flow cytometer. [0003] Enzyme-linked immunosorbent assay has low sensitivity, time-consuming and cumbersome operation, which has certain limitations in the detection of exosomes. [0004] Ultracentrifugation is currently the most commonly used method for exosome purification. The number of exosomes obtained by simple operation is large, but the process is time-consuming and the recovery rate is unstable, and the purity is also questioned. cause damage, thereby reducing its quality. [0005]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N33/574
CPCB01L3/5027B01L2200/10G01N33/57488
Inventor 陈欲超
Owner SHENZHEN HUIXIN LIFE TECH CO LTD
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