Development and Application of Specific Probes for Tandem Repeat Sequences of Decaploid Echinopsis elongatum

A technology for S. longarum and sequence, which is applied in the field of crop genetics and breeding, can solve the problems of lack of complete genome sequence information, hindering the development and application of genetic resources of D. longarum, and inability to obtain chromosomal-specific molecular markers in batches, etc.
CN107475390BActive Publication Date: 2020-04-07INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Publication Date
2020-04-07

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Abstract

The invention discloses development and application of a decaploid agropyron elongatum tandem repeat sequence specific probe. According to the development and the application, a specific length amplified fragment sequencing technology (SLAF-seq) is utilized to perform sequencing on common wheat, a common wheat-decaploid agropyron elongatum translocation line and decaploid agropyron elongatum, a decaploid agropyron elongatum specific sequence is acquired through sequence data comparative analysis, and the tandem repeat sequence specific probe is developed based on the decaploid agropyron elongatum specific sequence. The tandem repeat sequence specific probe provided by the invention can be used for rapidly identifying decaploid agropyron elongatum chromosomes and identifying a translocation line in a process that chromosome fragments of agropyron elongatum are transferred into the wheat, providing an important way of molecular marker-assisted selection to improve the wheat hereditary property and also providing an important application basis for wheat molecular breeding, phyletic evolution and germplasm resource identification.
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Description

technical field

[0001] The invention belongs to the field of crop genetics and breeding, and in particular relates to the development and application of specific probes for the tandem repeat sequence of the decaploid Etyophony elongatum. Background technique

[0002] Wheat (Triticum aestivm) is the second largest food crop with the largest planting area in the world and its output is second only to corn. In the past half century, the total output of wheat in the world has more than tripled, and excellent wheat varieties have played a decisive role in increasing wheat output. There are a large number of beneficial genetic resources that wheat lacks in wild relatives of wheat, and fully developing and utilizing these excellent genetic resources to breed new wheat varieties has become one of the goals of wheat breeding. Thinopyrum ponticum (Thinopyrum ponticum, 2n=10x=70) is a perennial herb belonging to the genus Thinopyrum ponticum in the Poaceae family, and is an important ...

Claims

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