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Application of rykonic acid h derived from Penicillium oxalicum in the preparation of anti-human esophageal cancer drugs

A technology of ryeketonic acid and esophageal cancer, which is applied in the field of medicine and achieves the effects of significant human esophageal cancer proliferation activity and inhibition of human esophageal cancer proliferation activity

Active Publication Date: 2020-05-08
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that the indicated ryronic acid compounds have anti-human esophageal cancer activity, and there is no report on the anti-proliferation activity of this compound on human esophageal cancer cells, so there is no drug related to this in the market.

Method used

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  • Application of rykonic acid h derived from Penicillium oxalicum in the preparation of anti-human esophageal cancer drugs
  • Application of rykonic acid h derived from Penicillium oxalicum in the preparation of anti-human esophageal cancer drugs
  • Application of rykonic acid h derived from Penicillium oxalicum in the preparation of anti-human esophageal cancer drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Fermentative production and separation and purification of the compound of embodiment 1

[0020] 1 Fermentation production

[0021] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Penicillium oxalicum ( Penicillium oxalicum ) IBPT-6 (preserved in China Center for Type Culture Collection on December 25, 2013, address: Wuhan University, Wuhan, deposit number is: CCTCC NO: M 2013714) appropriate amount, inoculated on PDA solid slant medium at 28 Cultivate in an incubator for 3 days.

[0022] Penicillium oxalicum ( Penicillium oxalicum ) Appropriate amount of IBPT-6, inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5, MgSO 4 ·7H 2 O 0.3, NaCl 15.0 constant volume] in a 1000mL Erlenmeyer flask, after 30 days of static culture at 28°C, the mycelia and fermentation broth were ...

Embodiment 2

[0031] Example 2 Test of anti-tumor activity in vitro

[0032] 1 Experimental samples and experimental methods

[0033] Preparation of the tested sample solution The test sample is the pure product of the compound separated and refined in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0034] The subculture of cell lines and cells adopts tumor cell lines, and the tumor cells are treated with DMEM medium containing 10% FBS at 37°C with 5% CO 2 Subculture in an incubator.

[0035] Cell Proliferation Inhibitory Activity Test Method

[0036] Tetrazolium salt (MTT) method Take tumor cells in the logarithmic growth phase and adjust the cell density to 1×10 per ml 5 Cells were inoculated into 96-well cell culture plates at 200 microliters per well, and filled with 5% CO at 37°C 2for 4 hours in an incubator. Add 2 microliters of sample solution...

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Abstract

The present invention relates to applications of secalonic acid H derived from Penicillium oxalicum in preparation of anti-human esophageal cancer drugs. According to the present invention, the compound can inhibit the proliferation of esophageal cancer cells, and has the following structural formula defined in the specification; Penicillium oxalicum IBPT-6 is subjected to fermentation culture to obtain a fermentation product, and the fermentation product is separated and purified to obtain the compound; and the experiment results prove that the compound has good antitumor activity on human esophageal cancer cell EC9706, and can be used for preparing human esophageal cancer cell proliferation inhibition drugs or antitumor drugs, and can be used for human esophageal cancer research.

Description

technical field [0001] The invention relates to the application of ryeconic acid H derived from Penicillium oxalicum in the preparation of anti-human esophageal cancer drugs, and belongs to the field of medicine. Background technique [0002] Secalonic acids belong to the secondary metabolites of Ergochrome and are xanthone dimers. Since Stoll et al. isolated Secalonic acid A (Secalonic acid A) from fungi in 1952, the series of compounds Secalonic acid (A, B, C, D, E, F, G) have been continuously identified. discovery and research. Secalonic acid compounds have various physiological activities. Taking Secalonic acid D (Secalonic acidD, SAD) as an example, 5 mg / ml of SAD is added to normal saline, within the range of 5-20 mg, that is It can treat early bladder cancer, within the range of 50-100 mg, it is effective for more serious bladder cancer and has no side effects. It has been found through research that some marine fungi can produce ryryronic acid compounds with nove...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/80C12R1/80
CPCA61K31/352C12P17/06
Inventor 陈立鲁志浩夏其文刘沁颖毕延雪伍久林张其清
Owner FUZHOU UNIV
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