Application of LncRNA to preparation of lung adenocarcinoma diagnosing reagent

A technology of lung adenocarcinoma, CLDN10-AS1, applied in detection reagents, LncRNA in the field of preparing reagents for diagnosing lung adenocarcinoma, which can solve the problems of complicated and long-term detection of lung adenocarcinoma genes

Active Publication Date: 2017-12-19
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the above technical problems in the prior art, the present invention provides a use of LncRNA in the preparation of reagents for diagnosing lung adenocarc...

Method used

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  • Application of LncRNA to preparation of lung adenocarcinoma diagnosing reagent
  • Application of LncRNA to preparation of lung adenocarcinoma diagnosing reagent
  • Application of LncRNA to preparation of lung adenocarcinoma diagnosing reagent

Examples

Experimental program
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Effect test

Embodiment 1

[0022] 1. LncRNA expression data analysis in lung adenocarcinoma and normal lung tissues

[0023] First, the LncRNA sequencing data of lung adenocarcinoma and lung normal tissues were downloaded from the TCGA database, and a total of 488 lung adenocarcinoma tissues and 58 lung normal tissues were included. The expression level of lncRNA is represented by FPKM value.

[0024] LncRNAs that can be applied to the diagnosis of lung adenocarcinoma lung normal tissues were initially screened based on the following criteria.

[0025] (1) The multiple difference between lung adenocarcinoma and normal tissue is greater than 10.

[0026] (2) P<0.01.

[0027] (3) LncRNAs expressed in lung adenocarcinoma accounted for more than 80%.

[0028] We finally selected 6 LncRNAs and searched for different transcripts of LncRNAs through the Ensembl database. They all had more than one transcript. In order to improve the reliability of the results, DNAstar software was used to analyze their conse...

Embodiment 2

[0068] The use of embodiment 2 kit

[0069] With reference to the method of Example 1, total RNA was extracted from lung adenocarcinoma patient’s cancer tissue specimen and adjacent normal tissue, and after reverse transcription into cDNA, the diagnostic kit of the present invention was used to detect LINC00942, LINC00973, AFAP1-AS1 by RT-PCR , CLDN10-AS1 expression difference in lung adenocarcinoma and adjacent normal tissues.

[0070] The present invention collected surgical resection specimens from patients with primary lung adenocarcinoma in the Department of Thoracic Surgery of Zhongshan Hospital from February 2016 to December 2016. Using the kit of the present invention, the screened LncRNA was verified by QRT-PCR in surgically resected pathological specimens, and it was found that the expressions of LINC00942, LINC00973, AFAP1-AS1, and CLDN10-AS1 were significantly higher in cancer tissues than in paracancerous tissues.

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Abstract

The invention provides application of any one or more than two long non-coding RNAs to preparation of a lung adenocarcinoma diagnosing reagent. The LncRNA is LINC00942, LINC00973, AFAP1-AS1 or CLDN10-AS1, wherein the cDNA sequence of the LINC00942 long non-coding RNA is shown as SEQ ID NO.15; the cDNA sequence of the LINC00973 long non-coding RNA is shown as SEQ ID NO.16; the cDNA sequence of the AFAP1-AS1 long non-coding RNA is shown as SEQ ID NO.17; and the cDNA sequence of the CLDN10-AS1 long non-coding RNA is shown as SEQ ID NO.18. The LncRNA can be applied to lung adenocarcinoma diagnosis.

Description

technical field [0001] The invention belongs to the field of biological genes, and relates to a detection reagent, in particular to the use of LncRNA in the preparation of a reagent for diagnosing lung adenocarcinoma. Background technique [0002] Lung cancer has become the malignant tumor with the highest incidence rate. In 2015, there were 733,300 new cases, of which men ranked first, and women were second only to breast cancer. In 2015, 610,200 people died of lung cancer, and the cancer mortality rate was the highest among men and women. Both ranked first. The age-standardized incidence rate and age-standardized death rate of lung cancer remained stable in men, but continued to increase in women, and the cost of diagnosis and treatment related to lung cancer also increased significantly year by year. Therefore, the prevention and treatment of lung cancer has become one of the major health care problems in my country and even in the world. Lung cancer is divided into smal...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 方硕詹成范虹姚光宇张永星张毅杨晓冬谢晓枫沈小静
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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