Gas-liquid exchange device

Abstract

The invention relates to a novel gas supply device applicable to cell culture reactors, in particular to a gas-liquid exchange device comprising three parts, namely, a liquid storage bottle, a siphon and a circulating pipeline. The siphon is connected with a liquid outlet of the liquid storage bottle through a circulating hose, the lower end of the siphon is connected with an elbow, and the lower end of the elbow is connected with a liquid inlet of the liquid storage bottle through a hose. With the adoption of the gas-liquid exchange device, the probability of production of bubbles after gas enters the reactor can be reduced, the effects of bubble breakage on cells can be reduced, and the density of cells in the reactor can be increased.

Description

technical field [0001] The invention belongs to the field of cell culture devices, in particular to a gas-liquid exchange device. Background technique [0002] At present, the world's biotechnology pharmaceutical industry is developing rapidly and has made great achievements. The expression and production of various diagnostic and therapeutic monoclonal antibodies, vaccines, growth factors and other biologically active proteins through in vitro culture of animal cells has a very broad market application prospect. Therefore, bioreactors used for large-scale high-density cultivation of animal cells have become the most important equipment in the biotechnology industry. [0003] In the prior art, there are generally two ways to ventilate into the cell culture reactor: one is to directly ventilate into the reactor through a pipeline; the other is to set a closed pipeline in the reactor, and then Drill some small holes in the pipe to ventilate. This kind of pipe is also called ...

AI Technical Summary

Problems solved by technology

The above two ways of ventilating into the reactor are easy to generate bubbles in the reactor, and the rupture of the bubbles in the cell culture liquid will cause cell damage or even death, thereby affecting the cell density in the reactor

Images