Centrifugal separation immunochromatography detection method and device

A technology of centrifugal separation and immunochromatography, applied in the field of immunodetection, can solve problems such as inability to achieve simultaneous implementation, and achieve the effects of improving detection accuracy, improving capture and binding ability, and reducing background noise interference.

Inactive Publication Date: 2017-12-29
BEIJING KANGHUAYUAN SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

High sensitivity, rapidity, convenience, miniaturization, full quantification, and automation are the current development trends of clinical immunoassay technology products, but none of the existing ones can achieve the above functions at the same time

Method used

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  • Centrifugal separation immunochromatography detection method and device
  • Centrifugal separation immunochromatography detection method and device
  • Centrifugal separation immunochromatography detection method and device

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Experimental program
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Embodiment 1

[0036] Embodiment 1, the preparation of horizontal centrifugal separation detection device

[0037] Such as figure 1 As shown, the centrifuge detection device of the present invention includes: a sample feeding part 1 , a solid phase detection membrane 2 , a centrifugal device (a centrifugal rotor 3 ; a drive motor 5 ; a support base 6 ) and a detector 4 . It also includes ultrasonic breaking device 7 and casing 8 . The proximal end of the solid-phase detection membrane 2 corresponding to the sampling part 1, the ultrasonic breaking device 7 corresponds to the detection part of the solid-phase detection membrane 2, the centrifugal device is composed of a centrifugal rotor 3, a driving motor 5 and a supporting base 6, and the driving motor 5 Located on the supporting base 6 , the centrifugal rotor 3 is driven to rotate by a driving motor 5 .

[0038] Such as figure 1 with figure 2 As shown, the solid-phase detection membrane 2 is provided with a liquid adsorption and dispe...

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Abstract

The invention discloses a centrifugal separation immunochromatography detection method. Based on the characteristic that a centrifugal device is utilized for driving a liquid phase to flow in a solid-phase detection film based on an existing immunochromatography technique, the method can be applied to a colloid metal immunochromatography detection method, a fluorescence immunochromatography detection method and a chemiluminiscence immunochromatography detection method. The method has the characteristics of high sensitivity, short detection time, convenience in use, high stability and convenience in storage.

Description

technical field [0001] The invention relates to a centrifugal separation detection method and a device, belonging to the technical field of immune detection. Background technique [0002] Immunological detection technology is an experimental method designed by applying the principles of immunology to determine antigens, antibodies, immune cells and chemical components. Samples for analytical, food and industrial analysis. Commonly used immunoturbidity technology, solid-phase enzyme immunoassay technology, chemiluminescent detection technology, immunofluorescence labeling technology, flow cytometry, colloidal gold technology, etc. Immunoturbidimetry, also known as immunoturbidimetry, is the specific combination of soluble antigens and antibodies in the liquid phase to produce complexes of a certain size, which form light refraction or absorption, and measure the transmitted or scattered light after refraction or absorption. As a calculation unit, it is used for quantitative...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543G01N33/68
CPCG01N33/558G01N33/54313G01N33/6887
Inventor 刘凤鸣
Owner BEIJING KANGHUAYUAN SCI TECH CO LTD
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