Stable biological stain and preparation method of same

A biological staining agent and a stable technology, applied in the field of chemical staining agents, can solve problems such as difficult to see structural features, inaccurate analysis, and poor accuracy of results, and achieve good coloring effect, clear color, and high integrity

Inactive Publication Date: 2018-01-19
郑州乐业生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing commonly used staining methods are highly subjective, which can easily lead to inaccurate analysis, and most tissues have high water content, strong light transmission, tenderness, and soft texture, which are difficult to see clearly by direct naked eye or stereoscopic observation. Its structural characteristics, the accuracy of the results is poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In the embodiment of the present invention, a stable biological dye is composed of the following raw materials according to mass percentage: 1% trinitrophenol, 30% sodium persulfate, 3% acrylate, 15% medical grade acetic acid, 5,6 ,7,8-tetrahydrofolate 0.5%, horseradish peroxidase 5%, formalin 1%, ethanol 30%, the balance is deionized water.

[0022] The mass content of formaldehyde in formalin is 1%.

[0023] Its preparation method comprises the following steps:

[0024] 1) Mix trinitrophenol, sodium persulfate, medical grade acetic acid and deionized water thoroughly, let it settle for 1 hour, take the supernatant, and filter it with qualitative filter paper to obtain mixture A.

[0025] 2) Mix mixture A with acrylate, 5,6,7,8-tetrahydrofolate, horseradish peroxidase, and ethanol evenly, place it in a water bath at 30°C for 5 minutes, and then heat it in a water bath Add formalin during the process; stir evenly at a stirring speed of 60r / min to obtain a biological d...

Embodiment 2

[0027] In the embodiment of the present invention, a stable biological dye is composed of the following raw materials according to mass percentage: 15% trinitrophenol, 2% sodium persulfate, 25% acrylate, 1% medical grade acetic acid, 5,6 ,7,8-tetrahydrofolate 15%, horseradish peroxidase 0.1%, formalin 10%, ethanol 1%, and the balance is deionized water.

[0028] The mass content of formaldehyde in formalin is 5%.

[0029] Its preparation method comprises the following steps:

[0030] 1) Mix trinitrophenol, sodium persulfate, medical grade acetic acid and deionized water thoroughly, let it settle for 24 hours, take the supernatant, and filter it with qualitative filter paper to obtain mixture A.

[0031] 2) Mix mixture A with acrylate, 5,6,7,8-tetrahydrofolate, horseradish peroxidase, and ethanol evenly, place it in a water bath at 80°C for 50 minutes, and then heat it in a water bath Add formalin during the process; stir evenly at a stirring speed of 300r / min to obtain a bio...

Embodiment 3

[0033] In the embodiment of the present invention, a stable biological stain is composed of the following raw materials according to mass percentage: 2% trinitrophenol, 25% sodium persulfate, 5% acrylate, 12% medical grade acetic acid, 5,6 ,7,8-tetrahydrofolate 1%, horseradish peroxidase 4%, formalin 2%, ethanol 25%, and the balance is deionized water.

[0034] The mass content of formaldehyde in formalin is 2%.

[0035] Its preparation method comprises the following steps:

[0036] 1) Mix trinitrophenol, sodium persulfate, medical grade acetic acid and deionized water thoroughly, let it settle for 4 hours, take the supernatant, and filter it with qualitative filter paper to obtain mixture A.

[0037] 2) Mix mixture A with acrylate, 5,6,7,8-tetrahydrofolate, horseradish peroxidase, and ethanol evenly, place it in a water bath at 40°C for 8 minutes, and then heat it in a water bath Add formalin during the process; stir evenly at a stirring speed of 100r / min to obtain a biolog...

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PUM

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Abstract

The invention discloses a stable biological stain and a preparation method of same. The biological stain is composed of, by mass, 1-15% of trinitrophenol, 2-30% of sodium persulfate, 3-25% of acrylate, 1-15% of medical-grade acetic acid, 0.5-15% of 5,6,7,8-tetrahydrofolic acid, 0.1-5% of horseradish peroxidase, 1-10% of formalin, 1-30% of ethanol, and the balanced being deionized water. The preparation method includes the steps of: 1) fully mixing the deionized water with the trinitrophenol, the sodium persulfate and the medical-grade acetic acid, allowing the mixture to stand for precipitating, and collecting a supernatant; 2) mixing the supernatant with the acrylate, the 5,6,7,8-tetrahydrofolic acid, the horseradish peroxidase and the ethanol, and heating the mixture at 30-80 DEG C in water bath for 5-50 min, during the water bath heating process, the formalin is added; and 3) uniformly stirring the mixture to obtain the biological stain. The biological stain is low in usage amount,has good staining effect, can form clear colors, is easy to elute in later processes, is free of changing hardness of a sample and is high in sample completeness. The biological stain allows the sample to be quickly, high-effectively and accurately displayed in anatomy of biomedicine.

Description

technical field [0001] The invention relates to the technical field of chemical dyes, in particular to a stable biological dye and a preparation method thereof. Background technique [0002] In recent years, with the development of medicine and the deepening of pathological research, human beings need a large number of anatomical experiments to analyze and detect for reference for further pathological analysis, so when performing biomedical anatomical experiments, staining samples can The anatomy is well observed. In general, biomedical dissection experiments focus on the analysis of tissue morphology, mainly by staining the entire tissue so that exfoliated tissue can be clearly observed under a microscope. The existing commonly used staining methods are highly subjective, which can easily lead to inaccurate analysis, and most tissues have high water content, strong light transmission, tenderness, and soft texture, which are difficult to see clearly by direct naked eye or s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B67/44G01N1/30
Inventor 井政郑一超常婕
Owner 郑州乐业生物科技有限公司
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