Production method of cell block paraffin section for fully presenting position of malignant cell

Abstract

The invention discloses a production method of a cell block paraffin section for fully presenting the position of a malignant cell. The method comprises the following steps: firstly, acquiring a primary cell sample and performing centrifugation sediment on the primary cell sample, so as to obtain a secondary cell sample; after the secondary cell sample is fixed, placing the secondary cell sample into a tissue dehydration box in a manner of vertical dividing from the middle for dehydration, transparentizing and waxing in sequence, so as to obtain a cell fixed sample; and vertically placing thecell fixed sampled into a tissue embedding box for embedding by taking a plane of section as a bottom surface, so as to form the section finally. Each section produced by the method can present the same cell hierarchical structure, so that the malignant cell appears at the same position of each section, a phenomenon that the malignant cell is located unreliably and even disappears in continuous sections is avoided, a comparative analysis when various antibodies are labeled in immunocytochemistry is facilitated, and cytopathology doctors can be effectively assisted to judge the source and the type of a malignant tumor, and therefore, the accuracy rate of cytopathological diagnosis is improved, a clinical application effect is good, and the practical value is high.

Description

technical field [0001] The invention belongs to the technical field of cytopathology, and in particular relates to a method for making a cell block paraffin section that completely presents the position of malignant tumor cells. Background technique [0002] Cytopathology is a discipline that diagnoses and studies clinical diseases by observing the structural and morphological changes of cells. In clinical pathological diagnosis, common cytological samples include serous cavity effusion, sputum, urine, various lavage fluids, cervical cells and fine needle aspiration specimens, etc. The high accuracy of positive results makes cytopathological examination an important means for disease screening and tumor diagnosis. In the process of clinical diagnosis, the preparation method of common cytopathological smears is to directly smear cells on glass slides or smear after centrifugation, and observe under a microscope after fixation, Papanicolaou staining or hematoxylin-eosin stain...

AI Technical Summary

Problems solved by technology

However, in the traditional method of making paraffin-embedded sections, the cell block is placed horizontally in the tissue embedding box for embedding. The cell components are different. When a specific cell needs to be observed, the location of the specific cell may be unreliable or even disappear in consecutive slices.

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