Production method of cell block paraffin section for fully presenting position of malignant cell

A technology for malignant tumors and paraffin sections, applied in the field of cytopathology, can solve the problems of disappearance and unreliable cell location, etc., and achieve simple steps, easy promotion and use, and good results

Inactive Publication Date: 2018-02-09
杨莉
View PDF7 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the traditional method of making paraffin-embedded sections, the cell block is placed horizontally in the tissue embedding box for embedding. The ...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Production method of cell block paraffin section for fully presenting position of malignant cell
  • Production method of cell block paraffin section for fully presenting position of malignant cell
  • Production method of cell block paraffin section for fully presenting position of malignant cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0037] Such as figure 1 The method for making a cell block paraffin section that completely presents the location of malignant tumor cells, the specific steps are as follows:

[0038] Step 1. Obtain primary cell samples: put the collected cell liquid into a transparent, flat-bottomed container, perform pretreatment on the cell liquid to obtain primary cell samples, and place the primary cell samples in a centrifuge tube;

[0039] Preferably, the cell fluid in step 1 is a bloody liquid, and the pretreatment process of the bloody liquid is as follows: put the bloody liquid into a transparent, flat-bottomed container, and then place the container containing the bloody liquid at 4°C. After standing in the refrigerator for 15h-24h, use a straw to collect the gray-white sediment on the surface of the red blood cells at the bottom of the container as the primary cell sample.

[0040] Preferably, the cell fluid in step 1 is a bloody liquid, and the pretreatment process of the bloody ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a production method of a cell block paraffin section for fully presenting the position of a malignant cell. The method comprises the following steps: firstly, acquiring a primary cell sample and performing centrifugation sediment on the primary cell sample, so as to obtain a secondary cell sample; after the secondary cell sample is fixed, placing the secondary cell sample into a tissue dehydration box in a manner of vertical dividing from the middle for dehydration, transparentizing and waxing in sequence, so as to obtain a cell fixed sample; and vertically placing thecell fixed sampled into a tissue embedding box for embedding by taking a plane of section as a bottom surface, so as to form the section finally. Each section produced by the method can present the same cell hierarchical structure, so that the malignant cell appears at the same position of each section, a phenomenon that the malignant cell is located unreliably and even disappears in continuous sections is avoided, a comparative analysis when various antibodies are labeled in immunocytochemistry is facilitated, and cytopathology doctors can be effectively assisted to judge the source and the type of a malignant tumor, and therefore, the accuracy rate of cytopathological diagnosis is improved, a clinical application effect is good, and the practical value is high.

Description

technical field [0001] The invention belongs to the technical field of cytopathology, and in particular relates to a method for making a cell block paraffin section that completely presents the position of malignant tumor cells. Background technique [0002] Cytopathology is a discipline that diagnoses and studies clinical diseases by observing the structural and morphological changes of cells. In clinical pathological diagnosis, common cytological samples include serous cavity effusion, sputum, urine, various lavage fluids, cervical cells and fine needle aspiration specimens, etc. The high accuracy of positive results makes cytopathological examination an important means for disease screening and tumor diagnosis. In the process of clinical diagnosis, the preparation method of common cytopathological smears is to directly smear cells on glass slides or smear after centrifugation, and observe under a microscope after fixation, Papanicolaou staining or hematoxylin-eosin stain...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N1/28G01N1/36
CPCG01N1/286G01N1/36G01N2001/2873
Inventor 杨莉王映梅付欣杨巧马娟蔡梦霞赵建业
Owner 杨莉
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap