Method for degrading and modifying schizophyllan by using self-produced endonuclease of schizophyllum commune Fr. fermentation system

A technology of schizophyllan and schizophyllan, which is applied in the field of schizophyllan, can solve the problems of poor hydrolysis efficiency, long hydrolysis time, and high price, and achieve the effects of increasing yield, simplifying the process, and reducing production costs

Active Publication Date: 2018-02-13
GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, there are not many commercialized endo-β-1,3 glucanases at present, the price is relatively expensive, and generally the activity is low, the hydrolysis efficiency is poor, the hydrolysis time is long, the hydrolysis process is not easy to control, and the hydrolyzate Mainly small molecules

Method used

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  • Method for degrading and modifying schizophyllan by using self-produced endonuclease of schizophyllum commune Fr. fermentation system
  • Method for degrading and modifying schizophyllan by using self-produced endonuclease of schizophyllum commune Fr. fermentation system
  • Method for degrading and modifying schizophyllan by using self-produced endonuclease of schizophyllum commune Fr. fermentation system

Examples

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Comparison scheme
Effect test

Embodiment 1

[0026] figure 1 It is the GPC elution curve of enzymatic degradation proschiphyllan under the conditions of Example 1;

[0027] figure 2 It is the GPC elution curve of Schizophyllan after enzymatic degradation under the conditions of Example 1;

Embodiment 2

[0028] image 3 It is the GPC elution curve of the enzymatic degradation pro-schizophyllan under the conditions of Example 2;

[0029] Figure 4 It is the GPC elution curve of Schizophyllan after enzymatic degradation under the conditions of Example 2;

Embodiment 3

[0030] Figure 5 It is the GPC elution curve of the enzymatic degradation pro-schizophyllan under the conditions of Example 3;

[0031] Figure 6 It is the GPC elution curve of Schizophyllan after enzymatic degradation under the conditions of Example 3;

[0032] Figure 7 It is a comparison chart of the solubility of schizophyllan after enzymatic degradation and that of schizophyllan before enzymatic hydrolysis under the conditions of Examples 1, 2, and 3.

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Abstract

The invention discloses a method for degrading and modifying schizophyllan by using self-produced endonuclease of a schizophyllum commune Fr. fermentation system. The method comprises the steps of firstly, carrying out revaccination, activation and culture on a slant strain of schizophyllum commune Fr. by using a PDA culture medium to obtain fermented seed liquid; inoculating in the fermented seedliquid, continuously culturing and fermenting under the conditions that the stirring speed is 100-250rpm, the ventilation rate is 0.2-0.7vvm and the culture temperature is 26-29 DEG C; then, carryingout ceramic microfiltration membrane separation, modifying polysaccharide in filtrate by means of enzymolysis, and separating the modified polysaccharide to obtain the modified polysaccharide. Afterthe method is adopted, the molecular weight of the modified polysaccharide is reduced to 1500kDa or less from 4000kDa or more by means of degradation, and the modified polysaccharide can be completelydissolved in distilled water within 24h. The method provided by the invention utilizes enzyme secreted by the strain to degrade the polysaccharide, thus not only solving the problem that specific enzyme is difficult to obtain, but also greatly simplifying the technology and lowering the production cost; therefore, the method for obtaining the soluble polysaccharide has industrial potential.

Description

technical field [0001] The present invention relates to schizophyllan, in particular to a method for enzymatically modifying fermented polysaccharides by utilizing the self-produced enzyme of the schizophyllum fermentation system; the obtained schizophyllan has suitable molecular weight and good solubility, and can be applied to biomedicine and health care products, cosmetics and other industries. Background technique [0002] Schizophyllum commune Fr. is a very important edible and medicinal fungus, which is rich in physiologically active substances. Schizophyllan (SPG) is a water-soluble polysaccharide extracted from the fruiting body, mycelium or fermentation broth of Schizophyllum fungus. β-D-glucan as a side chain. SPG has a variety of physiological activities such as tumor inhibition, antibacterial and anti-inflammatory, anti-radiation, and improvement of body immunity. However, due to the large molecular weight and poor water solubility of schizophyllan, it is not ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04C12R1/645
CPCC12P19/04C12P19/14
Inventor 郑必胜邓娜周林刘瑞海裴运林
Owner GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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