Efficient separation and purification method for F2-generation chili hybrid variety

Abstract

The invention discloses an efficient separation and purification method for F2-generation chili hybrid variety. The efficient separation and purification method comprises the steps of culturing a chili seedling, cultivating the chili seedling, and carrying out descendant screening. The expanding propagation and acceleration generation are carried out through soilless cultivation of a chili matrix,the front four generations are not screened, and at least four generations can be cultivated in one year, so that the material purification time is greatly shortened, and the purification cost is lowered; a fifth generation is cultivated through pot culture, so that the characters of a chili material is adequately expressed; a contrast is added, more than 95% of intermediate plants are removed byvirtue of a two-end selection method; and a sixth generation is subjected to ground screening in a field, and high-homozygous chili material with target character can be screened. The efficient separation and purification method is simple in operation, short in time and high in efficiency, a large amount of cost is saved, and the screened chili material is high in purity and good in character.

Description

technical field [0001] The invention relates to the field of purification of capsicum parent materials, in particular to a high-efficiency separation and purification method for the F2 generation of capsicum hybrids. Background technique [0002] Pepper is a fresh vegetable and condiment that Chinese people like to eat. It is rich in nutrition and has various eating methods. It is widely cultivated all over the country and has a long history of cultivation. Capsicum belongs to monoecious and often outcrossing crops. Under natural conditions, self-pollination is the main method, but cross-pollination also occurs frequently. The natural outcrossing rate of each variety is about 16.5% when there is no isolation. The heterosis of pepper is very obvious, and the yield of fine combinations is 30%-40% higher than that of traditional main varieties. At present, most of the fine combinations of peppers used in production are the first generation of hybrids. The key to the utilizatio...

AI Technical Summary

Problems solved by technology

The purification process of the offspring is mainly based on multiplication and generation in the field. Each generation needs to be separated and screened once. Screening 1-2 generations a year usually takes more than 4 years to obtain 6 generations of high-purity self-breeding system, consuming a lot of manpower, material and financial resources

With the popularization of soilless culture technology, some people also use soilless culture of pepper as an auxiliary breeding method in the multiplication and generation of pepper parents, but the related soilless culture management technology is not mature enough. Excellent traits cannot be expressed in the process of isolation and screening

For example, capsicum is mainly cultivated in the field and has a high yield. When using soilless cultivation, because the cultivation management technology cannot be compared with that in the field for the time being, the final output is relatively lower than that of field cultivation. Therefore, in terms of yield In terms of traits, it is difficult to screen out parent materials that meet the requirements of high yield