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Avian influenza virus infection or avian influenza virus vaccine immunophenotyping kit

An avian influenza virus and avian influenza technology, applied in biological testing, analytical materials, instruments, etc., can solve the problems of affecting egg production, high lethality, and mass death of chickens

Active Publication Date: 2018-03-06
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, for chickens, the H5 subtype is highly pathogenic, which will lead to the death of chickens in batches; the H7 subtype has a high lethality rate, and the infection has been increasing in recent years; although the H9 subtype is low pathogenicity, But it will significantly affect the egg production of laying hens

Method used

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  • Avian influenza virus infection or avian influenza virus vaccine immunophenotyping kit
  • Avian influenza virus infection or avian influenza virus vaccine immunophenotyping kit
  • Avian influenza virus infection or avian influenza virus vaccine immunophenotyping kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0093] 1. Preparation and Confirmation of Peptides

[0094] The polypeptides used in the examples were synthesized by Jill Biochemical (Shanghai) Co., Ltd. and confirmed by mass spectrometry. in,

[0095] SEQ ID NO: 1: LMENERTLDFHDSNVKNLYD.

[0096] SEQ ID NO: 2: QQFELIDNEFNEVEKQIGNV.

[0097] SEQ ID NO: 3: ADSEMDKLYERVKRQLRENA.

[0098] SEQ ID NO: 4: FHKCDDDCMASIRNNTYDHR.

[0099] 2. Preparation of kit (detection chip)

[0100] Spot the solutions of the polypeptides shown in the above SEQ ID NO: 1 to 4 on the conventional ELISA solid phase carrier by conventional methods, and spot a chicken IgY spot as a positive quality control spot and a PB spot as a negative quality control spot point, the detection chip was prepared.

[0101] 3. Detection with kit

[0102] Inspection steps

[0103] (1) Before starting the test, dilute the concentrated cleaning solution with purified water or distilled water at a ratio of 1:10, and use it directly after dilution. Use a pipet...

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Abstract

The invention relates to an avian influenza virus infection or avian influenza virus vaccine immunophenotyping kit. The detection kit disclosed by the invention comprises one or a plurality of solid carriers and a specific polypeptide assembly which is independently connected to the one or the plurality of the solid carriers.

Description

technical field [0001] The invention mainly relates to a poultry kit and a diagnostic method. In particular, the present invention relates to a kit and a diagnostic method that can be used for typing of avian influenza virus infection and / or avian influenza vaccine immunity. Background technique [0002] Avian influenza is a disease syndrome of poultry and wild birds caused by type A influenza virus. The disease was first discovered in Italy in 1878. It was called "fowl plague" at that time. It was not confirmed until 1955 that it was infected by the H7 strain of influenza A virus. [0003] In recent years, chicken avian influenza has been on the rise in countries all over the world, especially the outbreak of avian influenza in Hong Kong since 1997 has threatened human health. Chicken avian influenza has hotspots such as acute onset, widespread prevalence, rapid death, and great harm. It is a first-class infectious disease listed by the International Veterinary Office (OI...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/68G01N33/535
Inventor 马宏伟
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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