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An SSR molecular marker used for indentifying germplasm resources of polygonatum Mill., and applications thereof

A technology of molecular markers and Polygonatum, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of low sequencing error rate and non-use, etc., and achieve high application value, high polymorphism, good reproducibility

Active Publication Date: 2018-04-10
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

[0005] SLAF-seq (Specific-Locus Amplified Fragment Sequencing) technology is an effective sequencing method based on simplified representative library sequencing (RRLs) and high-throughput paired-end sequencing. It has the characteristics of high throughput, low sequencing error rate, and low cost. , has been widely used in the development of molecular markers in plants in recent years, but this method has not been applied to the development of SSR markers in Polygonatum.

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  • An SSR molecular marker used for indentifying germplasm resources of polygonatum Mill., and applications thereof
  • An SSR molecular marker used for indentifying germplasm resources of polygonatum Mill., and applications thereof
  • An SSR molecular marker used for indentifying germplasm resources of polygonatum Mill., and applications thereof

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Embodiment 1

[0047] Example 1 Acquisition of SSR Molecular Markers for Identification of Polygonatum Germplasm and Its Application in Identifying Different Varieties of Polygonatum

[0048] The SSR molecular marker used to identify Polygonatum Mill. (Polygonatum Mill.) germplasm of the present invention is obtained by the following method:

[0049] 1. Select 24 Polygonatum species from 21 regions in 11 provinces of the country, including Polygonatum multiflora, Polygonatum and Polygonatum, see Table 1. The DNA samples extracted from them were sent to Beijing Biomark Biotechnology Co., Ltd., and the samples were resequenced using SLAF-seq technology. In the present invention, the sequencing sequence is used to mine specific SSR sites, and 48 pairs of Polygonatum SSR primers are designed, and the primers are synthesized by Beijing Liuhe Huada Gene Technology Co., Ltd.

[0050] Table 1 24 germplasm materials of Polygonatum and their molecular IDs

[0051]

[0052]

[0053] 2. The proc...

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Abstract

The invention provides an SSR molecular marker used for indentifying germplasm resources of polygonatum Mill., and applications thereof, and belongs to the technical field of germplasm resource identification assisted by plant molecular markers. The SSR molecular marker is one or a plurality of molecular markers selected from the following seven SSR molecular markers. The seven SSR molecular markers are obtained by amplification through primers shown as SEQ ID NO.1-14. The provided SSR molecular marker can differentiate different germplasm resources of polygonatum Mill., with operation being simple, repeatability being good and accuracy being high. The SSR molecular marker can effectively overcome a defect that different germplasm resources of polygonatum Mill. cannot be accurately differentiated by utilizing phenotype identification methods, and is of great significance for polygonatum Mill. germplasm resource identification and molecular maker assisted selection.

Description

technical field [0001] The invention relates to the technical field of identification of germplasm resources assisted by plant molecular markers, in particular to SSR molecular markers for identifying germplasm resources of the genus Polygonatum and its application. Background technique [0002] Polygonatum Mill. is a perennial herb of Liliaceae (Liliaceae). The rhizome is horizontal, cylindrical, and nodules are enlarged. It is often used as medicine with dry rhizome. There are more than 40 species of this genus in the world, and about 31 species in my country. Among them, Polygonatum Polygonatum, Polygonatum Polyflora and Polygonatum Dianensis are the "original medicines" of three Polygonatum Polygonatum included in the Pharmacopoeia of the People's Republic of China. Modern pharmacological studies have shown that Polygonatum has anti-aging effects, hypoglycemic and immune regulation effects, improving learning and memory abilities, anti-arteriosclerosis and effects on th...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 邓欣
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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