Method for identifying Akebia trifoliata confusable variety based on tubular molecule characteristics under SEM (scanning electron microscopy)

Abstract

The invention discloses a method for identifying an Akebia trifoliata confusable variety based on tubular molecule characteristics under a SEM (scanning electron microscopy), and belongs to the technical field of identification of traditional Chinese medicinal materials. According to the method, an Akebia trifoliata stem is cut into thin segments, the thin segments are soaked in ethanol and treated with a chromic acid and nitric acid mixture in a 40 DEG C incubator; the treated material is washed with water, and centrifuged, a sediment is taken and preserved in ethanol and dyed with a safranine solution, and statistics on tubular molecule perforated plate type, pit membrane characteristics and aperture length can be performed under the SEM. The method can quickly and accurately identify Akebia trifoliata from ranunculaceous caulis clematidis armandii and Caulis aristolochiae manshuriensis. The significance of the method is to establish a directive standard for identification of micromorphology of commonly used akebiaquinata medicinal materials.

Description

technical field [0001] The invention belongs to the technical field of identification of traditional Chinese medicinal materials, and relates to an identification method of Akebia trifoliate and easily confused varieties thereof, in particular to an identification method of Akebia trifoliate easily confused varieties based on tubular molecular characteristics under SEM. Background technique [0002] The traditional Chinese medicine Akebia is derived from the roots and stems of Akebia clover and Akebia trifoliata, which have the functions of promoting blood circulation, relieving pain, clearing heat and diuresis. The traditional Chinese medicine Akebia, also known as Akebia, is recorded in the "Chinese Pharmacopoeia". However, in the history of medication, due to the shortage of Akebia resources, the plants of this genus belong to the historical mixed use, and the phenomenon of mixed use of Akebia ever occurred. The biggest, it will lead to severe renal failure, uremia, and s...

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Problems solved by technology

[0003] The traditional identification method of Akebia is mainly based on the shape of its stem (such as the roughness of the section, the diameter of the vessel) and the color of the powder (brown) (Pharmacopoeia of the People's Republic of China, 2010 edition), but due to the lack of detailed and objective standards, the identification is uncertain. accurate or false

In recent years, relatively new identification methods such as DNA barcodes, molecular markers, and chromatography are used for identification. DNA barcodes and molecular markers are expensive, long-term, and complex in data analysis, which increases the difficulty and cost of identification.

Due to differences in effective medicinal ingredients in different regions, different growth periods and different organs, it is difficult to determine a unified standard for comparison and screening

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