Method for freezing and preserving donkey seminal fluid

A technology for cryopreservation and semen, applied in the biological field, can solve the problems of low sperm motility rate and artificial insemination fertilization rate, etc., and achieve the effect of improving sperm motility rate

Inactive Publication Date: 2018-05-04
TIANJIN AGRICULTURE COLLEGE
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  • Abstract
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Problems solved by technology

The storage of semen includes room temperature storage (15-25°C), low temperature storage (4°C) and cryopreservation (-196°C or -79°C). Among them, only cryopreservatio

Method used

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Embodiment Construction

[0022] The implementation of the present invention will be described in further detail below. The following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0023] A method for cryopreservation of donkey semen, the method comprising steps as follows:

[0024] (1) Configure diluent

[0025] ① Base liquid configuration: Accurately weigh 3.04g of Tris, 1.70g of citric acid, 1.50g of glucose, 1.50g of fructose, 0.06g of penicillin, and 0.10g of streptomycin with an electronic balance, and add 100mL of double distilled water to constant volume to make the base liquid;

[0026] ② Dilute Ⅰ solution configuration: use a 20mL sterile syringe to draw a total of 85mL of the base solution into a 100mL sterilized volumetric flask, use a 20mL sterile syringe to remove the needle, slowly add fresh egg yolk liquid into the volumetric flask, and set the volume to 100mL. uniform;

[0027] ③Preparation of dilution II solution: Us...

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Abstract

The invention relates to a method for freezing and preserving donkey seminal fluid. The method comprises the following steps: preparing a diluent; processing fresh seminal fluid; cooling, balancing, freezing, and unfreezing. A composition, which has a certain concentration and is composed of graphene oxide and glycerin, is added into a freezing liquid to prevent harms caused by formation of ice crystals on sperms; before the seminal fluid is frozen, the seminal fluid is cooled for 20-40 minutes by an ultra low temperature refrigerator with a temperature of -80 DEG C in advance; the difficultythat in a conventional method, seminal fluid should be cooled by a pricy programmed freezing instrument is solved; after freezing, the frozen seminal fluid is unfrozen by a water bath with a temperature of 60 DEG C; the living rate of sperms is effectively improved after the freeze preservation of donkey seminal fluid, and the method is suitable for artificial insemination of donkeys in grass-rootorganizations.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for cryopreserving donkey semen. Background technique [0002] There are about 10 million donkeys in my country, more than 30 species, mainly distributed in Shaanxi, Henan, Shandong, Xining, Gansu and other regions. In recent years, the number of donkeys in my country has continued to decline. The main reason is that most of the industrialized farming in China is mainly pigs, cattle, and sheep. There are few donkey breed resources and low reproductive efficiency. The purpose of semen preservation is to maximize the survival time of sperm and maintain their fertilization ability. The preserved semen is transported between different regions, which can expand the scope of use of semen, increase the number of mated animals, improve the utilization efficiency of breeding males, and fully exert the reproductive potential and genetic advantages of excellent breeding males. At pre...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 刘新峰郭宏丁向彬李新张林林
Owner TIANJIN AGRICULTURE COLLEGE
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