Animal semen diluting powder and application thereof
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A semen, animal technology, applied in the application, preservation of human or animal body, animal husbandry, etc., can solve the problems of poor semen preservation effect, low conception rate, short sperm survival time, etc., to improve antioxidant capacity, easy to preserve , Save the effect of good effect
Inactive Publication Date: 2020-09-15
洛宁农本畜牧科技开发有限公司
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Problems solved by technology
Although semen dilution has been applied for many years, there are still problems in actual production such as short sperm survival time, poor semen preservation effect, and low conception rate after artificial insemination.
Method used
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Embodiment 1
[0016] Pig semen dilution powder, the formula composition is as follows: liquiritin 0.1g, sodium glutamate 1.0g, L-arginine 1.0g, N-acetylcysteine 0.1g, fructose 1g, glucose 10g, potassium citrate 4g, Potassium chloride 0.5g, EDTA 2g, sodium carbonate 2g, oregano oil 0.1g, the formula dilution powder and PBS buffer are prepared into 1L dilution solution, and the pH value is adjusted to 6.8.
Embodiment 2
[0018] Pig semen dilution powder, the formula composition is as follows: Liquiritin 0.2g, Sodium Glutamate 2.0g, L-Arginine 1.0g, N-Acetyl Cysteine 0.2g, Fructose 2g, Glucose 12g, Potassium Citrate 3g, Potassium chloride 1g, EDTA 2g, sodium carbonate 2g, oregano oil 0.2g, the formula dilution powder and PBS buffer are prepared into 1L dilution solution, and the pH value is adjusted to 6.8.
Embodiment 3
[0020] Pig semen dilution powder, the formula composition is as follows: liquiritin 0.3g, sodium glutamate 3.0g, L-arginine 0.5g, N-acetylcysteine 0.3g, fructose 3g, glucose 15g, potassium citrate 2g, Potassium chloride 1g, EDTA 1g, sodium carbonate 1g, oregano oil 0.3g, the formula dilution powder and PBS buffer are prepared into a 1L dilution solution, and the pH value is adjusted to 6.8.
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Abstract
The invention relates to an animal semen diluting powder and application thereof, and belongs to the technical field of animal reproduction. The animal semen diluting powder is dissolved in a PBS buffer solution to prepare a pig semen diluting solution; the semen diluting solution comprises 0.1-0.3 g / L of liquiritin, 1-3 g of sodium glutamate, 0.5-1.0 g of L-arginine, 0.1-0.3 g of N-acetylcysteine, 1-3 g of fructose, 10-15 g of glucose, 2-4 g of potassium citrate, 0.5-1 g of potassium chloride, 1-2 g of EDTA, 1-2 g of sodium carbonate and 0.1-0.3 g of origanum oil. The liquiritin can also be replaced with glycyrrhetinic acid. The animal semen diluting powder does not adopt any antibiotics, and various components have a synergistic effect, so that the time and the motility rate of preservedsperms can be obviously improved, the integrity rate of acrosomes can be improved, and the pregnancy rate of artificial insemination after dilution can be increased.
Description
technical field [0001] The invention belongs to the technical field of animal reproduction, and in particular relates to an animal semen dilution powder and application thereof. Background technique [0002] With the continuous increase of population and the improvement of urbanization level, the change of residents' consumption concept and lifestyle and the improvement of income level, the consumption of pork continues to grow. Data from the National Bureau of Statistics shows that from 2009 to 2016, the per capita pork consumption of urban households in the country was basically stable at around 20.7kg / person. Artificial insemination technology is widely used in livestock and poultry production worldwide, and it can achieve reproductive results similar to natural mating. For example, currently, in most industrialized countries, 80% to 98% of sows are inseminated by artificial insemination techniques. Moreover, this ratio is also increasing year by year in other countries...
Claims
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Application Information
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