A method for creating cotton cytoplasmic male sterile lines by utilizing denucleated kapok

A technology of male sterile lines and male sterility, which is applied in the fields of botany equipment and methods, application, plant gene improvement, etc., can solve the problems that have not been reported, and achieve the effect of reducing risk and low seed production cost

Active Publication Date: 2021-03-30
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports at home and abroad on the research on the selection of cotton cytoplasmic male sterile lines by using combined-nucleated kapok or de-nucleated kapok

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Breeding of "16A in C5"

[0033]Using the denucleated kapok (provided by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences) as the cytoplasm donor and Zhongmian 16 (provided by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences) as the nucleus donor (recurrent parent), the saturated backcross method was used to select and breed Out of the cytoplasmic male sterile line of kapok kapok. Specifically:

[0034] a. hybridization

[0035] Taking Kapok kapok as the female parent and crossing with Zhongmian 16 to obtain the hybrid F 1 substitute seeds;

[0036] b. Backcross

[0037] F 1 Represents a fertile, crossed F 1 The fertile plants in the generation were continuously selfed for 2 generations, and male sterile plants appeared in the offspring, and the found sterile plants were used as the female parent, and Zhongmian 16 was used as the male parent for backcrossing, and all the offspring populations of ...

Embodiment 2

[0038] Example 2: Breeding of "40A in C5"

[0039] Two male sterile plants were found in the second generation of self-bred kapok kapok. Using the male sterile plant as the female parent and Zhongmian 40 (introduced by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences) as the recurrent parent, the cytoplasmic male sterile line "C5 Zhong 40A" and its Keep the line "C5 Zhong 40B". Specifically:

[0040] a. hybridization

[0041] Using the sterile plant found after selfing away from the denucleated kapok as the female parent, it was crossed with Zhongmian 40 to obtain the hybrid F 1 substitute seeds;

[0042] b. Backcross

[0043] Due to the above hybrid F 1 All the plants of this generation showed male sterility, so Zhongmian 40 was used as the male parent for backcrossing, and all the offspring populations of the backcross showed male sterility, indicating that the male sterility characteristic was cytoplasmic inheritance, so Zhongmian 40 was u...

Embodiment 3

[0044] Example 3: Backcross breeding of "C5 New 21A"

[0045] Taking the male sterile plant of the first generation of backcross (it can be any generation) in the breeding process of "16A in C5" in Example 1 as the female parent, and the sea-island cotton cultivar Xinhai 21 (introduced by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences) as the female parent. The male parent was backcrossed, and the cytoplasmic male sterile line "C5xin 21A" and its maintainer line "C5xin 21B" were bred through 6 generations of backcrossing.

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PUM

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Abstract

The invention discloses a method for creating cytoplasmic male sterile line of cotton by utilizing freestone gossypium barbadende L. The method comprises the following steps: taking freestone gossypium barbadende L or a sterile plant obtained by selfing of freestone gossypium barbadende L as parent to hybrid with cotton cultivars, if any generation of offspring obtained by hybridization, selfing and backcross all represent male sterility, indicating that the male sterility characteristic represents as cytoplasmic genetic characteristic, then taking the cultivars as recurrent parent to performnucleus replacement backcross till obtaining a cytoplasmic male sterile line with characteristics similar to that of the parent, and conformity and showing male sterility, wherein the recurrent parentis a corresponding male sterility maintainer line. The method of the invention has the advantages that Kaiyuan freestone gossypium barbadende L, or the hybrid or backcross offspring inheriting Kaiyuan freestone gossypium barbadende L cytoplasm is taken as a cytoplasm donor, and cultivars are served as nuclear donors, so that resulted cytoplasm-nucleus-interaction causes male sterility.

Description

technical field [0001] The invention relates to a crop breeding method, in particular to a method for creating a cotton cytoplasmic male sterile line by utilizing denucleated kapok. Background technique [0002] Cotton, Malvaceae, is one of the most important crops in the world. It is not only the most important fiber crop, but also an important oil crop, a high-protein food crop, textile, fine chemical raw material and an important strategic material. Cotton exhibits strong heterosis, and its heterosis rate is as high as 20%-30%. For a long time, the utilization of cotton heterosis has been an important content of cotton breeding at home and abroad. [0003] Cotton can use both nuclear male sterile lines and cytoplasmic male sterile lines to produce hybrid seeds. Although the use of nuclear male sterile lines is easy to find restorer lines and assemble strong dominant hybrid combinations, there are problems in the reproduction of sterile lines. Generally, male sterile st...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 周瑞阳
Owner GUANGXI UNIV
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