A method for creating cotton cytoplasmic male sterile lines by utilizing denucleated kapok
A technology of male sterile lines and male sterility, which is applied in the fields of botany equipment and methods, application, plant gene improvement, etc., can solve the problems that have not been reported, and achieve the effect of reducing risk and low seed production cost
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Embodiment 1
[0032] Example 1: Breeding of "16A in C5"
[0033]Using the denucleated kapok (provided by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences) as the cytoplasm donor and Zhongmian 16 (provided by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences) as the nucleus donor (recurrent parent), the saturated backcross method was used to select and breed Out of the cytoplasmic male sterile line of kapok kapok. Specifically:
[0034] a. hybridization
[0035] Taking Kapok kapok as the female parent and crossing with Zhongmian 16 to obtain the hybrid F 1 substitute seeds;
[0036] b. Backcross
[0037] F 1 Represents a fertile, crossed F 1 The fertile plants in the generation were continuously selfed for 2 generations, and male sterile plants appeared in the offspring, and the found sterile plants were used as the female parent, and Zhongmian 16 was used as the male parent for backcrossing, and all the offspring populations of ...
Embodiment 2
[0038] Example 2: Breeding of "40A in C5"
[0039] Two male sterile plants were found in the second generation of self-bred kapok kapok. Using the male sterile plant as the female parent and Zhongmian 40 (introduced by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences) as the recurrent parent, the cytoplasmic male sterile line "C5 Zhong 40A" and its Keep the line "C5 Zhong 40B". Specifically:
[0040] a. hybridization
[0041] Using the sterile plant found after selfing away from the denucleated kapok as the female parent, it was crossed with Zhongmian 40 to obtain the hybrid F 1 substitute seeds;
[0042] b. Backcross
[0043] Due to the above hybrid F 1 All the plants of this generation showed male sterility, so Zhongmian 40 was used as the male parent for backcrossing, and all the offspring populations of the backcross showed male sterility, indicating that the male sterility characteristic was cytoplasmic inheritance, so Zhongmian 40 was u...
Embodiment 3
[0044] Example 3: Backcross breeding of "C5 New 21A"
[0045] Taking the male sterile plant of the first generation of backcross (it can be any generation) in the breeding process of "16A in C5" in Example 1 as the female parent, and the sea-island cotton cultivar Xinhai 21 (introduced by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences) as the female parent. The male parent was backcrossed, and the cytoplasmic male sterile line "C5xin 21A" and its maintainer line "C5xin 21B" were bred through 6 generations of backcrossing.
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