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A method for creating a cotton cytoplasmic male sterile line by utilizing kapok kapok

A technology of male sterile lines and male sterility, which is applied in the fields of botany equipment and methods, application, plant gene improvement, etc., can solve the problems that have not been reported, and achieve the effect of reducing risk and low seed production cost

Active Publication Date: 2021-03-30
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports at home and abroad on the research on the selection of cotton cytoplasmic male sterile lines by using combined-nucleated kapok or de-nucleated kapok

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Breeding of "16A in C6"

[0035] Using Kaiyuanlian nuclear kapok (provided by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences) as the cytoplasm donor, and Zhongmian 16 (provided by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences) as the nucleus donor (recurrent parent), the saturated backcross method was used to select The cytoplasmic male sterile line of Kaiyuanlian nuclear kapok was bred. Specifically:

[0036] a. hybridization

[0037] Using Kaiyuan Lianhe kapok as the female parent, cross with Zhongmian 16 to obtain the hybrid F 1 substitute seeds;

[0038] b. Backcross

[0039] F 1 All the offspring are fertile, and the 1 The fertile plants in the generation were continuously selfed for 2 generations, and male sterile plants appeared in the offspring, and the found sterile plants were used as the female parent, and Zhongmian 16 was used as the male parent for backcrossing, and all the offs...

Embodiment 2

[0040] Example 2: Breeding of "40A in C6"

[0041] Using Kaiyuanlianhe kapok as material, 2 male sterile plants were found in the second generation of selfing. Using the male sterile plant as the female parent and Zhongmian 40 (introduced by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences) as the recurrent parent, the cytoplasmic male sterile line "C6 Zhong 40A" and its Keep the line "C6 Zhong 40B". Specifically:

[0042] a. hybridization

[0043] The sterile plant found after selfing of Kaiyuanlian nuclear kapok was used as the female parent, and it was crossed with Zhongmian 40 to obtain the hybrid F 1 substitute seeds;

[0044] b. Backcross

[0045] Due to the above hybrid F 1 All the plants of this generation showed male sterility, so Zhongmian 40 was used as the male parent for backcrossing, and all the offspring populations of the backcross showed male sterility, indicating that the male sterility characteristic was cytoplasmic inherit...

Embodiment 3

[0046] Example 3: Backcross breeding of "C6 New 21A"

[0047] Taking the male sterile plant of the first generation of backcross (it can be any generation) in the breeding process of "16A in C6" in Example 1 as the female parent, and the sea-island cotton cultivar Xinhai 21 (introduced by the Cotton Research Institute of the Chinese Academy of Agricultural Sciences) as the female parent. The male parent was backcrossed, and the cytoplasmic male sterile line "C6 Xin 21A" and its maintainer line "C6 Xin 21B" were bred through 6 generations of back crossing.

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Abstract

The invention provides a method for creating a cytoplasmic male sterile line of cotton by using perennial sea-island cotton. The perennial sea-island cotton or a sterile plant obtained from the perennial sea-island cotton through selfing is used as a parent to hybridize with a cotton cultivar, if any one generation of hybrid, selfing or backcross generations presents to be male sterile, the situation indicates that the male sterile feature is cytoplasmic inheritance, the cultivar is used as a recurrent parent for nuclear replacement backcrossing until the cytoplasmic male sterile line having similar, neat and consistent characters with the male parent and showing male sterility is obtained, and the recurrent parent is the corresponding male sterile maintainer line. According to the method,Kaiyuan perennial sea-island cotton or hybrid or backcross generations inherited from the Kaiyuan perennial sea-island cotton cytoplasm are taken as cytoplasmic donors, common cultivars are taken asnuclear donors, and the produced cytoplasm-nucleus interaction leads to male sterility.

Description

technical field [0001] The invention relates to a crop breeding method, in particular to a method for creating a cotton cytoplasmic male sterile line by using kapok kapok. Background technique [0002] Cotton, Malvaceae, is one of the most important crops in the world. It is not only the most important fiber crop, but also an important oil crop, a high-protein food crop, textile, fine chemical raw material and an important strategic material. Cotton exhibits strong heterosis, and its heterosis rate is as high as 20%-30%. For a long time, the utilization of cotton heterosis has been an important content of cotton breeding at home and abroad. [0003] Cotton can use both nuclear male sterile lines and cytoplasmic male sterile lines to produce hybrid seeds. Although the use of nuclear male sterile lines is easy to find restorer lines and assemble strong dominant hybrid combinations, there are problems in the reproduction of sterile lines. Generally, male sterile strains (gen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 周瑞阳
Owner GUANGXI UNIV
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