A method for male sterile line selection using the sterile cytoplasm of kenaf kn142
A KN142, male sterile line technology, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problem of single cytoplasmic source of kenaf hybrids, and reduce the risk of a certain disease. low cost effect
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Embodiment 1
[0024] Example 1 Breeding sterile lines with wild kenaf KN142
[0025] Taking the wild kenaf species KN142 as the female parent and Yuefeng No. 1 B (see: Example 3 of the National Invention Patent ZL200510019454.4) as the male parent, the method of saturated backcross was used to select the male sterile cytoplasm of KN142. The sterile line K4P3A and its maintainer line K4P3B.
[0026] details as follows:
[0027] From 2008 to 2010, crossed with KN142 as the female parent and Yuefeng No. 1 B as the male parent, and then backcrossed with Yuefeng No. 1 B as the male parent. At the same time of backcrossing, Fuhong 992 and R1 were used as the father. In this cross, the combining ability was determined. In each generation, the male sterile plants with high combining ability, similar traits to the male parent, and no anther dehiscence continued to be backcrossed, while the male parent was self-crossed. Backcrossing was carried out in Nanning, Guangxi and Lingshui, Hainan every yea...
Embodiment 2
[0028] Example 2 The sterile plant obtained by propagating offspring of KN142 seeds is used for transgenic sterile line
[0029] The seeds of natural pollination of wild kenaf species KN142 were harvested, and the seeds were planted to reproduce offspring, and sterile plants were obtained by screening. Taking the obtained sterile plant as the female parent and Yuefeng No. 1 B (see: Example 3 of the National Invention Patent ZL200510019454.4) as the male parent, the method of saturated backcrossing was used to select and breed the male sterile cytoplasm of KN142. The sterile line K4TP3A and its maintainer line K4TP3B.
[0030] details as follows:
[0031] From 2010 to 2012, the male sterile plant found in KN142 seed breeding offspring was used as the female parent, and Yuefeng No. 1B was used as the male parent to cross, and then backcrossed with Yuefeng No. 1B as the male parent. At the same time, Fuhong 992 and R1 were crossed as male parents to measure combining ability. I...
Embodiment 3
[0032] Example 3 Breeding sterile lines with wild kenaf KN142
[0033] Taking the wild kenaf species KN142 as the female parent and the conventional kenaf cultivar Jinguang Wuthorn (introduced from Fujian Agriculture and Forestry University) as the male parent, a sterile line with the cytoplasm of the KN142 male sterile line was selected by the method of saturation backcrossing. K4K3A and its maintainer K4K3B.
[0034] details as follows:
[0035] From 2010 to 2012, KN142 was used as the female parent, and Jinguang Wuthorn was used as the male parent to cross, and then backcross was performed with Jinguang Wuthorn as the male parent. At the same time of backcrossing, Fuhong 992 and R1 were crossed as the male parent, and the determination of Combining ability, select male sterile plants with high combining ability, similar traits to the male parent, and no anther dehiscence in each generation to continue backcrossing, while the male parent is self-crossed. Backcrossing was c...
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